Genome location: Pf3D7_14_v3:2,360,336..2,362,691(-)
Genome classification: Core
Product Description: vacuolar protein sorting-associated protein 4
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0016887 | ATP hydrolysis activity |
| Process | GO:0006886 | intracellular protein transport |
| Process | GO:0007033 | vacuole organization |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.04 | 0.98 |
| Trophozoite | expressed | 0.26 | 0.83 |
| Schizont | expressed | 0.36 | 0.77 |
| Gametocyte | expressed | 0.20 | 0.88 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1457500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -3.019 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PY17X_1325000)
Modification: Tagged | RMgm-1571
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKQ5
"Best" AlphaFill ligand hit: AGS (phosphothiophosphoric acid-adenylate ester, Local RMSD=0.14) with 1SXJ (Global RMSD=9.44)
No associated EC numbersOrthology to BindingDB Entries:
Ortholog Group (OrthoMCL): OG6_100670
Most Similar Human Ortholog: O75351
TM-align score: 0.76 | RMSD: 3.01
Seq Identity: 0.46 | Length: 348 / 419
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000119541 | vacuolar protein sorting 4 homolog B |
| ENSG00000132612 | vacuolar protein sorting 4 homolog A |
| ENSG00000260914 | novel protein |
Protein Length: 419 | Molecular Weight (kDa): 48.259
UniProt ID(s): Q8IKQ5
PDB ID(s): None
Isoelectric Point: 8.28
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR003959 | ATPase, AAA-type, core |
| InterPro | IPR007330 | MIT |
| InterPro | IPR015415 | Vps4 oligomerisation, C-terminal |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR036181 | MIT domain superfamily |
| InterPro | IPR041569 | AAA ATPase, AAA+ lid domain |
| PFam | PF00004 | ATPase, AAA-type, core |
| PFam | PF04212 | MIT |
| PFam | PF09336 | Vps4 oligomerisation, C-terminal |
| PFam | PF17862 | AAA ATPase, AAA+ lid domain |
| Superfamily | SSF116846 | MIT domain superfamily |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 23 | 15 | 29 |
| disruptive | 1 | 14 | 18 | 52 |
| missense | 1 | 14 | 18 | 50 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 47 | 15 | 31 |
| disruptive | 4 | 59 | 59 | 81 |
| missense | 2 | 47 | 51 | 64 |
PlasmoDB Total SNPs: 149
Non-coding: 124 | Synonymous: 22 | Nonsynonymous: 3 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 15252121 | The Plasmodium falciparum Vps4 homolog mediates multivesicular body formation. | Yang M, Coppens I, ..., Joiner KA | 10.1242/jcs.01237 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |