PF3D7_1458300

Genome location: Pf3D7_14_v3:2,391,113..2,397,128(-)

Genome classification: Core

Function and Localization

Product Description: alpha/beta hydrolase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 3

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.07	0.96
Trophozoite	expressed	0.62	0.67
Schizont	expressed	1.28	0.38
Gametocyte	expressed	0.39	0.77

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1458300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.324 | #Insertions: 7

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1322000)

Relative Growth Rate: 0.94 ± 0.13
Confidence: 5.42

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1322000)

Modification: Disrupted | RMgm-3512

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IKP8

"Best" AlphaFill ligand hit: IFM (afegostat, Local RMSD=0.08) with 7LOU (Global RMSD=23.29)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533517

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	8	94	56	129
disruptive	33	228	108	288
missense	30	223	108	267

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	16	189	86	127
disruptive	68	469	193	372
missense	54	424	176	298

PlasmoDB Total SNPs: 221

Non-coding: 186 | Synonymous: 25 | Nonsynonymous: 10 | Stop Codon: 0

Protein Information

Protein Length: 1528 | Molecular Weight (kDa): 183.546

UniProt ID(s): Q8IKP8

PDB ID(s): None

Isoelectric Point: 8.56

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR029058	Alpha/Beta hydrolase fold
PFam	N/A	N/A
Superfamily	SSF53474	Alpha/Beta hydrolase fold

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104