Genome location: Pf3D7_14_v3:2,397,349..2,399,312(-)
Genome classification: Core
Product Description: aminodeoxychorismate lyase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 4.1.3.38 (Aminodeoxychorismate lyase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Function | GO:0008696 | 4-amino-4-deoxychorismate lyase activity |
| Function | GO:0004084 | branched-chain-amino-acid transaminase activity |
| Function | GO:0008483 | transaminase activity |
| Process | GO:0009082 | branched-chain amino acid biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.04 | 0.98 |
| Trophozoite | not expressed | 0.16 | 0.89 |
| Schizont | not expressed | 0.02 | 0.98 |
| Gametocyte | not expressed | 0.06 | 0.96 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1458400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.992 | MFS: -2.98 | #Insertions: 2
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1322100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1322100)
Modification: Tagged | RMgm-4597
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKP7
"Best" AlphaFill ligand hit: PMP (pyridoxamine-5'-phosphate, Local RMSD=0.33) with 1A0G (Global RMSD=2.88)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 4.1.3.38 | aminodeoxychorismate lyase | D-glutamate |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 11 | 9 | 24 |
| disruptive | 16 | 47 | 23 | 58 |
| missense | 16 | 47 | 23 | 52 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 25 | 21 | 14 |
| disruptive | 26 | 84 | 38 | 73 |
| missense | 26 | 78 | 33 | 48 |
PlasmoDB Total SNPs: 120
Non-coding: 90 | Synonymous: 5 | Nonsynonymous: 23 | Stop Codon: 2
Protein Length: 316 | Molecular Weight (kDa): 37.512
UniProt ID(s): Q8IKP7
PDB ID(s): None
Isoelectric Point: 7.65
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001544 | Aminotransferase class IV |
| InterPro | IPR036038 | Aminotransferase-like, PLP-dependent enzymes |
| PFam | PF01063 | Aminotransferase class IV |
| Superfamily | SSF56752 | Aminotransferase-like, PLP-dependent enzymes |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23957380 | Completing the folate biosynthesis pathway in Plasmodium falciparum:p-aminobenzoate is produced by a highly divergent promiscuousaminodeoxychorismate lyase. | Magnani G, Lomazzi M, Peracchi A | 10.1042/BJ20130896 |