Genome location: Pf3D7_14_v3:2,420,234..2,423,452(+)
Genome classification: Core
Product Description: ATP-dependent RNA helicase DBP5
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0010494 | cytoplasmic stress granule |
| Component | GO:0005634 | nucleus |
| Function | GO:0033679 | 3'-5' DNA/RNA helicase activity |
| Function | GO:0033678 | 5'-3' DNA/RNA helicase activity |
| Function | GO:0016887 | ATP hydrolysis activity |
| Function | GO:0003723 | RNA binding |
| Function | GO:0003724 | RNA helicase activity |
| Function | GO:0017116 | single-stranded DNA helicase activity |
| Process | GO:0032508 | DNA duplex unwinding |
| Process | GO:0016973 | poly(A)+ mRNA export from nucleus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.75 | 0.65 |
| Trophozoite | expressed | 0.57 | 0.67 |
| Schizont | expressed | 0.24 | 0.84 |
| Gametocyte | expressed | 0.43 | 0.75 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1459000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.13 | MFS: -2.887 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1322700)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKP1
"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.04) with 4KBF (Global RMSD=2.14)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.13 | RNA helicase | ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP... |
Orthology to BindingDB Entries:
Ortholog Group (OrthoMCL): OG6_101341
Most Similar Human Ortholog: I3L0H8
TM-align score: 0.80 | RMSD: 3.37
Seq Identity: 0.35 | Length: 396 / 741
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000109832 | DEAD-box helicase 25 |
| ENSG00000157349 | DEAD-box helicase 19B |
| ENSG00000168872 | DEAD-box helicase 19A |
| ENSG00000260537 | novel protein, DDX19B and DDX19A readthrough |
Protein Length: 741 | Molecular Weight (kDa): 84.314
UniProt ID(s): A6N5Z1, Q8IKP1, Q9GPH0
PDB ID(s): None
Isoelectric Point: 4.87
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 52 | 26 | 59 |
| disruptive | 13 | 84 | 41 | 157 |
| missense | 9 | 78 | 35 | 140 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 17 | 104 | 41 | 64 |
| disruptive | 31 | 213 | 106 | 196 |
| missense | 23 | 185 | 86 | 129 |
PlasmoDB Total SNPs: 119
Non-coding: 55 | Synonymous: 32 | Nonsynonymous: 32 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 21168450 | A novel dual Dbp5/DDX19 homologue from Plasmodium falciparum requires Q motif foractivity. | Mehta J, Tuteja R | 10.1016/j.molbiopara.2010.12.003 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 28500788 | Unraveling the importance of the malaria parasite helicases. | Tuteja R | 10.1111/febs.14109 |
| 35286399 | The nucleoporin Gle1 activates DEAD-box protein 5 (Dbp5) by promoting ATP bindingand accelerating rate limiting phosphate release | Gray S, Cao W, Montpetit B, De La Cruz EM | 10.1093/nar/gkac164 |
| 37436777 | The DEAD-box RNA helicase Dbp5 is a key protein that couples multiple steps ingene expression | Querl L, Krebber H | 10.1515/hsz-2023-0130 |
| 38189406 | Gle1 is required for tRNA to stimulate Dbp5 ATPase activity in vitro and promoteDbp5-mediated tRNA export in vivo in Saccharomyces cerevisiae | Arul Nambi Rajan A, Asada R, Montpetit B | 10.7554/eLife.89835 |
| 32969336 | Correction: A nuclear role for the DEAD-box protein Dbp5 in tRNA export | Lari A, Arul Nambi Rajan A, ..., Montpetit B | 10.7554/eLife.63264 |
| 31344122 | Correction: Depletion of mRNA export regulator DBP5/DDX19, GLE1 or IPPK that is akey enzyme for the production of IP6, resulting in differentially alteredcytoplasmic mRNA expression and specific cell defect | Okamura M, Yamanaka Y, ..., Masuda S | 10.1371/journal.pone.0220511 |
| 33205304 | Emerging molecular functions and novel roles for the DEAD-box protein Dbp5/DDX19in gene expression | Arul Nambi Rajan A, Montpetit B | 10.1007/s00018-020-03680-y |
| 37425677 | Gle1 is required for tRNA to stimulate Dbp5 ATPase activity in vitro and topromote Dbp5 mediated tRNA export in vivo | Rajan AAN, Asada R, Montpetit B | 10.1101/2023.06.29.547072 |
| 33002012 | Dbp5 associates with RNA-bound Mex67 and Nab2 and its localization at the nuclearpore complex is sufficient for mRNP export and cell viability | Adams RL, Wente SR | 10.1371/journal.pgen.1009033 |
| 35349720 | Correction to 'The nucleoporin Gle1 activates DEAD-box protein 5 (Dbp5) bypromoting ATP binding and accelerating rate limiting phosphate release' | 10.1093/nar/gkac221 | |
| 31453808 | A nuclear role for the DEAD-box protein Dbp5 in tRNA export | Lari A, Arul Nambi Rajan A, ..., Montpetit B | 10.7554/eLife.48410 |
| 32041247 | Dbp5/DDX19 between Translational Readthrough and Nonsense Mediated Decay | Beissel C, Grosse S, Krebber H | 10.3390/ijms21031085 |
| 35965007 | Attacking a DEAD problem: The role of DEAD-box ATPases in ribosome assembly and beyond | Karbstein K | 10.1016/bs.mie.2022.03.033 |