PF3D7_1459700

Genome location: Pf3D7_14_v3:2,444,059..2,446,716(-)

Genome classification: Core

Function and Localization

Product Description: pyridoxal 5'-phosphate synthase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 1.4.3.5 (Pyridoxal 5'-phosphate synthase);5.1.99.6 (NAD(P)H-hydrate epimerase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion
Function	GO:0052856	NADHX epimerase activity
Function	GO:0052857	NADPHX epimerase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.04	0.98
Trophozoite	expressed	0.39	0.76
Schizont	expressed	0.04	0.98
Gametocyte	expressed	0.18	0.89

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1459700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.393 | MFS: -3.104 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1323300)

Relative Growth Rate: 0.97 ± 0.11
Confidence: 5.87

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1323300)

Modification: Disrupted | RMgm-3518

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IKN4

"Best" AlphaFill ligand hit: TDR (thymine, Local RMSD=0.24) with 3RO7 (Global RMSD=1.63)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
1.4.3.5	pyridoxal 5'-phosphate synthase	Phenylglyoxal2,3-Butanedione2,4-pentandioneammonium sulfatediethyldicarbonatepyridoxal 5'-phosphate4-chloromercuribenzoatepyridoxine 5'-phosphatecitrate-phosphate bufferpyridoxamine 5'-phosphate4-Pyridoxic acid phosphatepyridoxaloxime 5'-phosphate...
5.1.99.6	NAD(P)H-hydrate epimerase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102975

Most Similar Human Ortholog: Q8NCW5

TM-align score: 0.76 | RMSD: 2.08

Seq Identity: 0.41 | Length: 231 / 287

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000163382	NAD(P)HX epimerase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	21	9	25
disruptive	1	37	32	57
missense	1	36	32	48

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	46	22	28
disruptive	7	80	46	91
missense	7	72	43	72

PlasmoDB Total SNPs: 186

Non-coding: 167 | Synonymous: 12 | Nonsynonymous: 7 | Stop Codon: 0

Protein Information

Protein Length: 287 | Molecular Weight (kDa): 32.96

UniProt ID(s): A0A144A6T9, Q8IKN4

PDB ID(s): None

Isoelectric Point: 9.81

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004443	YjeF N-terminal domain
InterPro	IPR032976	YjeF N-terminal domain-containing protein NAXE-like
InterPro	IPR036652	YjeF N-terminal domain superfamily
PFam	PF03853	YjeF N-terminal domain
Superfamily	SSF64153	YjeF N-terminal domain superfamily