Genome location: Pf3D7_14_v3:2,497,683..2,500,871(-)
Genome classification: Core
Product Description: splicing factor 3B subunit 2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005686 | U2 snRNP |
| Component | GO:0005684 | U2-type spliceosomal complex |
| Component | GO:0071013 | catalytic step 2 spliceosome |
| Component | GO:0005634 | nucleus |
| Component | GO:0071011 | precatalytic spliceosome |
| Component | GO:0005681 | spliceosomal complex |
| Process | GO:0000398 | mRNA splicing, via spliceosome |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.79 | 0.64 |
| Trophozoite | expressed | 0.79 | 0.57 |
| Schizont | expressed | 0.17 | 0.89 |
| Gametocyte | expressed | 0.48 | 0.74 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1461600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.394 | MFS: -2.549 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1325300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1325300)
Modification: Disrupted | RMgm-3524
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKL7
"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=1.02) with 3LFZ (Global RMSD=35.82)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102282
Most Similar Human Ortholog: E9PPJ0
TM-align score: 0.39 | RMSD: 4.62
Seq Identity: 0.41 | Length: 303 / 677
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000087365 | splicing factor 3b subunit 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 46 | 24 | 46 |
| disruptive | 16 | 86 | 51 | 124 |
| missense | 13 | 74 | 49 | 113 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 16 | 90 | 28 | 36 |
| disruptive | 34 | 189 | 95 | 171 |
| missense | 23 | 171 | 84 | 141 |
PlasmoDB Total SNPs: 169
Non-coding: 120 | Synonymous: 35 | Nonsynonymous: 14 | Stop Codon: 0
Protein Length: 677 | Molecular Weight (kDa): 79.339
UniProt ID(s): Q8IKL7
PDB ID(s): None
Isoelectric Point: 5.48
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006568 | PSP, proline-rich |
| InterPro | IPR007180 | Domain of unknown function DUF382 |
| PFam | PF04037 | Domain of unknown function DUF382 |
| PFam | PF04046 | PSP, proline-rich |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |