PF3D7_1461900

Genome location: Pf3D7_14_v3:2,514,219..2,518,607(+)

Genome classification: Core

Function and Localization

Product Description: valine--tRNA ligase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 6.1.1.9 (Valine--tRNA ligase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005829	cytosol
Function	GO:0004832	valine-tRNA ligase activity
Process	GO:0006418	tRNA aminoacylation for protein translation
Process	GO:0006438	valyl-tRNA aminoacylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.74	0.67
Trophozoite	expressed	0.99	0.50
Schizont	possibly expressed	0.11	0.93
Gametocyte	not expressed	0.27	0.85

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1461900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.123 | MFS: -3.064 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1325600)

Relative Growth Rate: 0.24 ± 0.20
Confidence: 4.62

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IKL5

"Best" AlphaFill ligand hit: TAR (d(-)-tartaric acid, Local RMSD=0.21) with 8C9D (Global RMSD=5.00)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
6.1.1.9	valine-tRNA ligase	KClarsenitetRNAVal(-CCA)Thr-tRNAValC76tRNAVal(-ACCA)L-Penicillamine1,10-phenanthrolineS-adenosylhomocysteinep-hydroxymercuribenzoateDL-2-amino-3-chlorobutyrateD-allo-2-Amino-3-chlorobutyratepyridoxal 5'-triphospho-5'-adenosine

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P26640	Valine--tRNA ligase	Homo sapiens	HOGENOM, OMA, OrthoDB	CHEMBL74755SB-203207CHEMBL421500

Orthology Information

Ortholog Group (OrthoMCL): OG6_100292

Most Similar Human Ortholog: P26640

TM-align score: 0.86 | RMSD: 2.07

Seq Identity: 0.44 | Length: 968 / 1090

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000096171	valyl-tRNA synthetase 1
ENSG00000137411	valyl-tRNA synthetase 2, mitochondrial
ENSG00000204394	valyl-tRNA synthetase 1
ENSG00000206476	valyl-tRNA synthetase 2, mitochondrial
ENSG00000223494	valyl-tRNA synthetase 2, mitochondrial
ENSG00000224264	valyl-tRNA synthetase 1
ENSG00000226589	valyl-tRNA synthetase 1
ENSG00000227686	valyl-tRNA synthetase 1
ENSG00000230985	valyl-tRNA synthetase 2, mitochondrial
ENSG00000231116	valyl-tRNA synthetase 1
ENSG00000231945	valyl-tRNA synthetase 1
ENSG00000234032	valyl-tRNA synthetase 2, mitochondrial
ENSG00000236178	valyl-tRNA synthetase 2, mitochondrial

Protein Information

Protein Length: 1090 | Molecular Weight (kDa): 128.071

UniProt ID(s): Q5KTV3, Q8IKL5

PDB ID(s): None

Isoelectric Point: 6.85

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001412	Aminoacyl-tRNA synthetase, class I, conserved site
InterPro	IPR002300	Aminoacyl-tRNA synthetase, class Ia
InterPro	IPR002303	Valine-tRNA ligase
InterPro	IPR009008	Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain
InterPro	IPR009080	Aminoacyl-tRNA synthetase, class Ia, anticodon-binding
InterPro	IPR013155	Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding
InterPro	IPR033705	Valyl tRNA synthetase, anticodon-binding domain
PFam	PF00133	Aminoacyl-tRNA synthetase, class Ia
PFam	PF08264	Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding
Superfamily	SSF47323	Aminoacyl-tRNA synthetase, class Ia, anticodon-binding
Superfamily	SSF50677	Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain
Superfamily	SSF52374

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	13	73	44	75
disruptive	23	118	64	199
missense	19	104	59	183

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	26	140	57	70
disruptive	46	273	124	283
missense	30	232	109	197

PlasmoDB Total SNPs: 144

Non-coding: 72 | Synonymous: 41 | Nonsynonymous: 30 | Stop Codon: 1