Genome location: Pf3D7_14_v3:2,558,055..2,560,979(+)
Genome classification: Core
Product Description: glyceraldehyde-3-phosphate dehydrogenase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 1.2.1.12 (Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0009986 | cell surface |
| Component | GO:0005829 | cytosol |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0020020 | food vacuole |
| Component | GO:0016020 | membrane |
| Component | GO:0005634 | nucleus |
| Function | GO:0003723 | RNA binding |
| Function | GO:0004365 | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0071466 | cellular response to xenobiotic stimulus |
| Process | GO:0044409 | entry into host |
| Process | GO:0006094 | gluconeogenesis |
| Process | GO:0006096 | glycolytic process |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.39 | 0.46 |
| Trophozoite | expressed | 2.69 | 0.09 |
| Schizont | expressed | 0.57 | 0.71 |
| Gametocyte | expressed | 0.92 | 0.56 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1462800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.132 | MFS: -2.858 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1326400)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKK7
"Best" AlphaFill ligand hit: MRY (meso-erythritol, Local RMSD=0.07) with 3PYM (Global RMSD=0.80)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.2.1.12 | glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P04406 | Glyceraldehyde-3-phosphate dehydrogenase | Homo sapiens | HOGENOM, OMA, OrthoDB | cid_1357397cid_2975202CHEMBL366942NAD+ analogue... |
| P46406 | Glyceraldehyde-3-phosphate dehydrogenase | Oryctolagus cuniculus | OrthoDB | 1-NITROSOCYCLOHEXYL TRIFLUOROACETATECHEMBL24192441-NITROSOCYCLOHEXYL ACETATEAngeli''s Salt |
Ortholog Group (OrthoMCL): OG6_100218
Most Similar Human Ortholog: P04406
TM-align score: 0.99 | RMSD: 0.65
Seq Identity: 0.65 | Length: 334 / 337
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000105679 | glyceraldehyde-3-phosphate dehydrogenase, spermatogenic |
| ENSG00000111640 | glyceraldehyde-3-phosphate dehydrogenase |
Protein Length: 337 | Molecular Weight (kDa): 36.635
UniProt ID(s): B0F3R3, O96369, Q8IKK7, Q8T6B1
PDB ID(s): None
Isoelectric Point: 7.65
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006424 | Glyceraldehyde-3-phosphate dehydrogenase, type I |
| InterPro | IPR020828 | Glyceraldehyde 3-phosphate dehydrogenase, NAD(P) binding domain |
| InterPro | IPR020829 | Glyceraldehyde 3-phosphate dehydrogenase, catalytic domain |
| InterPro | IPR020830 | Glyceraldehyde 3-phosphate dehydrogenase, active site |
| InterPro | IPR020831 | Glyceraldehyde/Erythrose phosphate dehydrogenase family |
| InterPro | IPR036291 | NAD(P)-binding domain superfamily |
| PFam | PF00044 | Glyceraldehyde 3-phosphate dehydrogenase, NAD(P) binding domain |
| PFam | PF02800 | Glyceraldehyde 3-phosphate dehydrogenase, catalytic domain |
| Superfamily | SSF51735 | NAD(P)-binding domain superfamily |
| Superfamily | SSF55347 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 23 | 9 | 23 |
| disruptive | 1 | 3 | 8 | 14 |
| missense | 1 | 2 | 7 | 14 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 47 | 10 | 19 |
| disruptive | 2 | 14 | 8 | 50 |
| missense | 1 | 12 | 8 | 39 |
PlasmoDB Total SNPs: 94
Non-coding: 85 | Synonymous: 8 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10767547 | Identification and recombinant expression of glyceraldehyde-3-phosphatedehydrogenase of Plasmodium falciparum. | Daubenberger CA, Poltl-Frank F, ..., Pluschke G | 10.1016/s0378-1119(00)00069-x |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 12974391 | The N'-terminal domain of glyceraldehyde-3-phosphate dehydrogenase of theapicomplexan Plasmodium falciparum mediates GTPase Rab2-dependent recruitment tomembranes. | Daubenberger CA, Tisdale EJ, ..., Pluschke G | 10.1515/BC.2003.135 |
| 16131754 | Structure of glyceraldehyde-3-phosphate dehydrogenase from Plasmodium falciparum. | Satchell JF, Malby RL, ..., Colman PM | 10.1107/S0907444905018317 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 16345073 | Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Plasmodiumfalciparum at 2.25 A resolution reveals intriguing extra electron density in theactive site. | Robien MA, Bosch J, ..., Hol WG | 10.1002/prot.20801 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 25137375 | Discovery of covalent inhibitors of glyceraldehyde-3-phosphate dehydrogenase, atarget for the treatment of malaria. | Bruno S, Pinto A, ..., Mozzarelli A | 10.1021/jm500747h |
| 26341815 | Cloning, expression, purification and characterization of Plasmodium spp.glyceraldehyde-3-phosphate dehydrogenase. | Sangolgi PB, Balaji C, ..., Jarori GK | 10.1016/j.pep.2015.08.028 |
| 26988354 | Analysis of the interactome of the Ser/Thr Protein Phosphatase type 1 inPlasmodium falciparum. | Hollin T, De Witte C, ..., Khalife J | 10.1186/s12864-016-2571-z |
| 27551151 | Identification of GAPDH on the surface of Plasmodium sporozoites as a newcandidate for targeting malaria liver invasion. | Cha SJ, Kim MS, Pandey A, Jacobs-Lorena M | 10.1084/jem.20160059 |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |