Genome location: Pf3D7_14_v3:2,565,034..2,566,641(-)
Genome classification: Core
Product Description: mediator of RNA polymerase II transcription subunit 20, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0016592 | mediator complex |
| Component | GO:0005634 | nucleus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.14 | 0.93 |
| Trophozoite | expressed | 0.25 | 0.84 |
| Schizont | expressed | 0.11 | 0.92 |
| Gametocyte | expressed | 0.40 | 0.77 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1463000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.271 | MFS: -3.409 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKK5
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 12 | 8 | 20 |
| disruptive | 2 | 3 | 5 | 17 |
| missense | 2 | 3 | 5 | 17 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 30 | 10 | 25 |
| disruptive | 3 | 19 | 31 | 70 |
| missense | 2 | 11 | 22 | 58 |
PlasmoDB Total SNPs: 69
Non-coding: 60 | Synonymous: 9 | Nonsynonymous: 0 | Stop Codon: 0
Protein Length: 308 | Molecular Weight (kDa): 36.71
UniProt ID(s): Q8IKK5
PDB ID(s): None
Isoelectric Point: 6.99
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | N/A | N/A |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 28288121 | A genetic system to study Plasmodium falciparum protein function. | Birnbaum J, Flemming S, ..., Spielmann T | 10.1038/nmeth.4223 |
| 23171760 | Mediator regulates non-coding RNA transcription at fission yeast centromeres | Thorsen M, Hansen H, ..., Thon G | 10.1186/1756-8935-5-19 |
| 26608234 | Loss of the Mediator subunit Med20 affects transcription of tRNA and othernon-coding RNA genes in fission yeast | Carlsten JO, Zhu X, ..., Gustafsson CM | 10.1016/j.bbagrm.2015.11.007 |