Genome location: Pf3D7_14_v3:2,569,896..2,572,178(+)
Genome classification: Core
Product Description: replication factor C subunit 3, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005663 | DNA replication factor C complex |
| Component | GO:0043626 | PCNA complex |
| Component | GO:0005634 | nucleus |
| Process | GO:0006281 | DNA repair |
| Process | GO:0006260 | DNA replication |
| Process | GO:0006271 | DNA strand elongation involved in DNA replication |
| Process | GO:0006261 | DNA-dependent DNA replication |
| Process | GO:0006272 | leading strand elongation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.03 | 0.99 |
| Trophozoite | expressed | 0.21 | 0.86 |
| Schizont | expressed | 0.04 | 0.97 |
| Gametocyte | expressed | 0.25 | 0.85 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1463200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.423 | MFS: -2.458 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1455100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKK4
"Best" AlphaFill ligand hit: 08T ([[[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium, Local RMSD=0.08) with 3U5Z (Global RMSD=3.15)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100321
Most Similar Human Ortholog: P40937
TM-align score: 0.92 | RMSD: 1.86
Seq Identity: 0.45 | Length: 332 / 344
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000049541 | replication factor C subunit 2 |
| ENSG00000111445 | replication factor C subunit 5 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 16 | 9 | 21 |
| disruptive | 0 | 8 | 6 | 27 |
| missense | 0 | 7 | 6 | 24 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 33 | 15 | 25 |
| disruptive | 0 | 26 | 26 | 77 |
| missense | 0 | 20 | 23 | 46 |
PlasmoDB Total SNPs: 84
Non-coding: 69 | Synonymous: 11 | Nonsynonymous: 4 | Stop Codon: 0
Protein Length: 344 | Molecular Weight (kDa): 39.232
PDB ID(s): None
Isoelectric Point: 7.05
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR003959 | ATPase, AAA-type, core |
| InterPro | IPR008921 | DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal |
| InterPro | IPR013748 | Replication factor C, C-terminal |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00004 | ATPase, AAA-type, core |
| PFam | PF08542 | Replication factor C, C-terminal |
| Superfamily | SSF48019 | DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 33040440 | Plasmodium falciparum replication factor C subunit 1 is involved in genotoxicstress response. | Sheriff O, Yaw A, ..., Preiser PR | 10.1111/cmi.13277 |
| 38059884 | Involvement of RFC3 in tamoxifen resistance in ER-positive breast cancer through the cell cycle | Zhu J, Ye L, ..., Zeng Z | 10.18632/aging.205260 |
| 37861747 | RFC3 serves as a novel prognostic biomarker and target for head and neck squamouscell carcinoma | Avs KR, Pandi C, ..., Arumugam P | 10.1007/s00784-023-05316-4 |