Product Description: DNA polymerase alpha subunit B, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.7 (DNA-directed DNA polymerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005658 | alpha DNA polymerase:primase complex |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0003887 | DNA-directed DNA polymerase activity |
| Process | GO:0071897 | DNA biosynthetic process |
| Process | GO:0006270 | DNA replication initiation |
| Process | GO:0006269 | DNA replication, synthesis of RNA primer |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.04 | 0.98 |
| Trophozoite | expressed | 0.38 | 0.76 |
| Schizont | expressed | 0.07 | 0.95 |
| Gametocyte | expressed | 0.22 | 0.87 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1463300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.135 | MFS: -3.065 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1326900)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKK3
"Best" AlphaFill ligand hit: UMP (2'-deoxyuridine 5'-monophosphate, Local RMSD=3.23) with 7S01 (Global RMSD=16.64)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.7 | DNA-directed DNA polymerase | KClEDTANH4+dATPNH4ClddTTPMK886UracilalliinsucroseethanolAllicin... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102459
Most Similar Human Ortholog: Q14181
TM-align score: 0.73 | RMSD: 3.31
Seq Identity: 0.19 | Length: 436 / 539
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000014138 | DNA polymerase alpha 2, accessory subunit |
| ENSG00000285816 | novel protein, POLA2-CDC42EP2 readthrough |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 28 | 16 | 35 |
| disruptive | 4 | 28 | 29 | 47 |
| missense | 4 | 27 | 29 | 42 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 50 | 20 | 35 |
| disruptive | 12 | 68 | 35 | 105 |
| missense | 9 | 57 | 30 | 83 |
PlasmoDB Total SNPs: 60
Non-coding: 26 | Synonymous: 21 | Nonsynonymous: 11 | Stop Codon: 2
Protein Length: 539 | Molecular Weight (kDa): 64.036
UniProt ID(s): Q8IKK3
PDB ID(s): None
Isoelectric Point: 6.62
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR007185 | DNA polymerase alpha/delta/epsilon, subunit B |
| InterPro | IPR016722 | DNA polymerase alpha, subunit B |
| PFam | PF04042 | DNA polymerase alpha/delta/epsilon, subunit B |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |