About Gene List

PF3D7_1463400

Genome location: Pf3D7_14_v3:2,574,832..2,576,807(+)

Genome classification: Core

Function and Localization

Product Description: DNA-directed RNA polymerase III subunit RPC4, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005666 RNA polymerase III complex

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring expressed 0.29 0.86
Trophozoite expressed 0.22 0.87
Schizont possibly expressed 0.03 0.98
Gametocyte possibly expressed 0.21 0.88

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1463400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.261 | MFS: -2.523 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1327000)

  • Relative Growth Rate: 0.26 ± 0.17
  • Confidence: 4.90

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IKK2

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
2.7.7.6 DNA-directed RNA polymerase BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_122843

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 0 12 14 24
disruptive 8 46 22 62
missense 8 46 20 54

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 1 33 21 29
disruptive 18 92 40 101
missense 15 76 36 74

PlasmoDB Total SNPs: 138

Non-coding: 116 | Synonymous: 12 | Nonsynonymous: 10 | Stop Codon: 0

Protein Information

Protein Length: 336 | Molecular Weight (kDa): 39.119

UniProt ID(s): Q8IKK2

PDB ID(s): None

Isoelectric Point: 9.3

Protein Domain Annotations:

Source Family ID Description
InterPro IPR007811 DNA-directed RNA polymerase III subunit RPC4
PFam PF05132 DNA-directed RNA polymerase III subunit RPC4
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104