Genome location: Pf3D7_14_v3:2,584,086..2,590,847(-)
Genome classification: Core
Product Description: rhoptry neck protein 11, putative
SignalP Peptide: MLMLYLHILLCIFLNNPVLVTN
# Transmembrane Domains: 7
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0044164 | host cell cytosol |
| Component | GO:1990225 | rhoptry neck |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.08 | 0.96 |
| Trophozoite | not expressed | 0.07 | 0.96 |
| Schizont | expressed | 1.99 | 0.27 |
| Gametocyte | not expressed | 0.07 | 0.96 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1463900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.155 | MFS: -2.846 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1327100)
Modification: Tagged | RMgm-4645
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKJ8
"Best" AlphaFill ligand hit: FAD (flavin-adenine dinucleotide, Local RMSD=22.24) with 3KLJ (Global RMSD=20.29)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 47 | 30 | 86 |
| disruptive | 29 | 109 | 52 | 172 |
| missense | 21 | 93 | 50 | 134 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 17 | 104 | 58 | 93 |
| disruptive | 68 | 224 | 117 | 276 |
| missense | 47 | 179 | 89 | 200 |
PlasmoDB Total SNPs: 428
Non-coding: 328 | Synonymous: 64 | Nonsynonymous: 36 | Stop Codon: 0
Protein Length: 1071 | Molecular Weight (kDa): 127.136
UniProt ID(s): Q8IKJ8
PDB ID(s): None
Isoelectric Point: 8.89
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR011992 | EF-hand domain pair |
| PFam | N/A | N/A |
| Superfamily | SSF47473 | EF-hand domain pair |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 18551176 | The malaria secretome: from algorithms to essential function in blood stageinfection. | van Ooij C, Tamez P, ..., Haldar K | https://pubmed.ncbi.nlm.nih.gov/18551176/ |
| 31247198 | Rhoptry neck protein 11 has crucial roles during malaria parasite sporozoiteinvasion of salivary glands and hepatocytes. | Bantuchai S, Nozaki M, ..., Ishino T | 10.1016/j.ijpara.2019.05.001 |
| 38334391 | PEXEL is a proteolytic maturation site for both exported and non-exportedPlasmodium proteins | Fierro MA, Muheljic A, ..., Beck JR | 10.1128/msphere.00393-23 |
| 38352500 | Plasmodium RON11 triggers biogenesis of the merozoite rhoptry pair and isessential for erythrocyte invasion | Anaguano D, Adewale-Fasoro O, ..., Muralidharan V | 10.1101/2024.01.29.577654 |
| 26950937 | Identification of Novel O-Linked Glycosylated Toxoplasma Proteins by Viciavillosa Lectin Chromatography | Wang K, Peng ED, ..., Bradley PJ | 10.1371/journal.pone.0150561 |