PF3D7_1464200 (ZFP)

Genome location: Pf3D7_14_v3:2,598,842..2,600,315(-)

Genome classification: Core

Function and Localization

Product Description: zinc finger CCCH domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.37	0.82
Trophozoite	not expressed	0.17	0.90
Schizont	not expressed	0.04	0.97
Gametocyte	not expressed	0.22	0.87

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1464200

Resistome Missense Mutations: N39K

Resistome Compounds with Missense Mutations: AN13762

Resistome # Samples with Disruptive Mutations: 2 (2 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.119 | MFS: -2.986 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1327600)

Relative Growth Rate: 0.06 ± 0.15
Confidence: 5.24

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IKJ5

"Best" AlphaFill ligand hit: PH2 (2-amino-6-hydroxymethyl-7,8-dihydro-3h-pteridin-4-one, Local RMSD=1.39) with 6JWR (Global RMSD=19.20)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_201707

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	13	13	17
disruptive	10	39	22	48
missense	9	38	22	44

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	34	22	18
disruptive	23	83	47	109
missense	18	74	36	73

PlasmoDB Total SNPs: 36

Non-coding: 17 | Synonymous: 7 | Nonsynonymous: 12 | Stop Codon: 0

Protein Information

Protein Length: 337 | Molecular Weight (kDa): 39.929

UniProt ID(s): Q8IKJ5

PDB ID(s): None

Isoelectric Point: 9.31

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000571	Zinc finger, CCCH-type
InterPro	IPR036855	Zinc finger, CCCH-type superfamily
PFam	PF00642	Zinc finger, CCCH-type
Superfamily	SSF90229	Zinc finger, CCCH-type superfamily

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
37887748	Functional Characterization of Eight Zinc Finger Motif-Containing Proteins inToxoplasma gondii Type I RH Strain Using the CRISPR-Cas9 System	Gao J, Wu XJ, ..., Zhu XQ	10.3390/pathogens12101232