Genome location: Pf3D7_14_v3:2,618,743..2,625,130(+)
Genome classification: Core
Product Description: serine/threonine protein phosphatase UIS2, putative
SignalP Peptide: MNILNLFICCLSITLLKFIEW
# Transmembrane Domains: 0
EC Numbers: 3.1.3.16 (Protein-serine/threonine phosphatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Component | GO:0020003 | symbiont-containing vacuole |
| Component | GO:0020005 | symbiont-containing vacuole membrane |
| Function | GO:0003993 | acid phosphatase activity |
| Function | GO:0008199 | ferric iron binding |
| Function | GO:0008198 | ferrous iron binding |
| Process | GO:0006470 | protein dephosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 2.29 | 0.21 |
| Trophozoite | expressed | 1.61 | 0.27 |
| Schizont | expressed | 0.92 | 0.59 |
| Gametocyte | expressed | 1.18 | 0.42 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1464600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.921 | MFS: -2.846 | #Insertions: 1
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1328000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1328000)
Modification: Tagged | RMgm-4418
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKJ1
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.3.16 | protein-serine/threonine phosphatase | ATPADPUDPAMPGTPUTPUMPNaFEDTAEGTAcAMPNADPH... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 84 | 49 | 101 |
| disruptive | 31 | 152 | 96 | 234 |
| missense | 28 | 147 | 89 | 209 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 26 | 162 | 54 | 109 |
| disruptive | 62 | 329 | 151 | 313 |
| missense | 50 | 292 | 133 | 229 |
PlasmoDB Total SNPs: 233
Non-coding: 145 | Synonymous: 48 | Nonsynonymous: 40 | Stop Codon: 0
Protein Length: 1442 | Molecular Weight (kDa): 170.48
UniProt ID(s): Q8IKJ1
PDB ID(s): None
Isoelectric Point: 5.41
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004843 | Calcineurin-like phosphoesterase domain, ApaH type |
| PFam | PF00149 | Calcineurin-like phosphoesterase domain, ApaH type |
| Superfamily | SSF56300 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 26735921 | UIS2: A Unique Phosphatase Required for the Development of Plasmodium LiverStages. | Zhang M, Mishra S, ..., Nussenzweig V | 10.1371/journal.ppat.1005370 |
| 28784333 | Identification of novel parasitophorous vacuole proteins in P. falciparumparasites using BioID. | Khosh-Naucke M, Becker J, ..., Spielmann T | 10.1016/j.ijmm.2017.07.007 |
| 29404413 | Bio ID Reveals Novel Proteins of the Plasmodium Parasitophorous Vacuole Membrane | Schnider CB, Bausch-Fluck D, ..., Burda PC | 10.1128/mSphere.00522-17 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 38334391 | PEXEL is a proteolytic maturation site for both exported and non-exportedPlasmodium proteins | Fierro MA, Muheljic A, ..., Beck JR | 10.1128/msphere.00393-23 |
| 37503245 | PEXEL is a proteolytic maturation site for both exported and non-exportedPlasmodium proteins | Fierro MA, Muheljic A, ..., Beck JR | 10.1101/2023.07.12.548774 |
| 28357358 | Translational Repression in Malaria Sporozoites | Turque O, Tsao T, Li T, Zhang M | 10.15698/mic2016.05.502 |