Genome location: Pf3D7_14_v3:2,640,139..2,642,202(-)
Genome classification: Core
Product Description: mediator of RNA polymerase II transcription subunit 4, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0016592 | mediator complex |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.30 | 0.85 |
| Trophozoite | expressed | 0.52 | 0.70 |
| Schizont | possibly expressed | 0.04 | 0.97 |
| Gametocyte | not expressed | 0.21 | 0.88 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1465200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.213 | MFS: -2.811 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1328600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKI6
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 13 | 13 | 20 |
| disruptive | 3 | 42 | 30 | 64 |
| missense | 3 | 40 | 30 | 61 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 31 | 18 | 31 |
| disruptive | 6 | 96 | 43 | 81 |
| missense | 5 | 87 | 42 | 67 |
PlasmoDB Total SNPs: 80
Non-coding: 72 | Synonymous: 5 | Nonsynonymous: 3 | Stop Codon: 0
Protein Length: 376 | Molecular Weight (kDa): 44.677
UniProt ID(s): Q8IKI6
PDB ID(s): None
Isoelectric Point: 5.89
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | N/A | N/A |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 35557691 | Mediator Complex of the Malaria Parasite Plasmodium falciparum Associates withEvolutionarily Novel Subunits. | Iyer UB, Park JE, ..., Featherstone M | 10.1021/acsomega.2c00368 |
| 37014863 | Yeast PIC-Mediator structure with RNA polymerase II C-terminal domain | Schilbach S, Wang H, Dienemann C, Cramer P | 10.1073/pnas.2220542120 |