PF3D7_1465300

Genome location: Pf3D7_14_v3:2,642,400..2,646,605(+)

Genome classification: Core

Function and Localization

Product Description: ribonuclease Z, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.26.11 (Ribonuclease Z)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion
Function	GO:0042781	3'-tRNA processing endoribonuclease activity
Process	GO:0072684	mitochondrial tRNA 3'-trailer cleavage, endonucleolytic
Process	GO:0034414	tRNA 3'-trailer cleavage, endonucleolytic

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.15	0.92
Trophozoite	expressed	0.24	0.85
Schizont	expressed	0.04	0.98
Gametocyte	expressed	0.28	0.83

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1465300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.231 | MFS: -3.33 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1328700)

Relative Growth Rate: 0.10 ± 0.12
Confidence: 5.60

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IKI5

"Best" AlphaFill ligand hit: T3F (thymidine-5'-diphosphate-alpha-d-3,6-dideoxy-3-aminogalactose, Local RMSD=8.41) with 7N7B (Global RMSD=29.44)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.26.11	tRNase Z	BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100263

Most Similar Human Ortholog: J3QRS2

TM-align score: 0.75 | RMSD: 3.15

Seq Identity: 0.20 | Length: 115 / 858

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000006744	elaC ribonuclease Z 2
ENSG00000141642	elaC ribonuclease Z 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	10	48	30	72
disruptive	23	123	87	178
missense	22	122	85	170

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	18	96	43	61
disruptive	48	267	120	213
missense	43	250	113	188

PlasmoDB Total SNPs: 264

Non-coding: 184 | Synonymous: 48 | Nonsynonymous: 32 | Stop Codon: 0

Protein Information

Protein Length: 858 | Molecular Weight (kDa): 100.044

UniProt ID(s): Q8IKI5

PDB ID(s): None

Isoelectric Point: 6.9

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001279	Metallo-beta-lactamase
InterPro	IPR036866	Ribonuclease Z/Hydroxyacylglutathione hydrolase-like
PFam	PF12706	Metallo-beta-lactamase
Superfamily	SSF56281	Ribonuclease Z/Hydroxyacylglutathione hydrolase-like