About Gene List

PF3D7_1465500 (K2)

Genome location: Pf3D7_14_v3:2,650,755..2,655,140(+)

Genome classification: Core

Function and Localization

Product Description: potassium channel K2

SignalP Peptide: N/A

# Transmembrane Domains: 7

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0016021 integral component of membrane
Component GO:0005886 plasma membrane
Function GO:0005267 potassium channel activity
Function GO:0005249 voltage-gated potassium channel activity
Process GO:0071805 potassium ion transmembrane transport

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.09 0.95
Trophozoite not expressed 0.03 0.98
Schizont not expressed 0.05 0.97
Gametocyte not expressed 0.34 0.81

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1465500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.34 | #Insertions: 6

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1328900)

  • Relative Growth Rate: 0.95 ± 0.10
  • Confidence: 5.99

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1328900)

Modification: Disrupted | RMgm-4315

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IKI3

"Best" AlphaFill ligand hit: R16 (hexadecane, Local RMSD=0.03) with 6W8F (Global RMSD=10.36)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_145632

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 5 81 40 101
disruptive 26 118 75 178
missense 10 108 68 151

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 15 161 65 111
disruptive 53 281 107 294
missense 23 216 90 208

PlasmoDB Total SNPs: 93

Non-coding: 0 | Synonymous: 55 | Nonsynonymous: 36 | Stop Codon: 2

Protein Information

Protein Length: 1461 | Molecular Weight (kDa): 174.221

UniProt ID(s): Q8IKI3

PDB ID(s): None

Isoelectric Point: 9.97

Protein Domain Annotations:

Source Family ID Description
InterPro IPR003929 Calcium-activated potassium channel BK, alpha subunit
InterPro IPR013099 Potassium channel domain
PFam PF03493 Calcium-activated potassium channel BK, alpha subunit
PFam PF07885 Potassium channel domain
Superfamily SSF81324 N/A

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
18218136 Characterization of two putative potassium channels in Plasmodium falciparum. Waller KL, McBride SM, Kim K, McDonald TV 10.1186/1475-2875-7-19
32957994 Purification and initial characterization of Plasmodium falciparum K(+) channels,PfKch1 and PfKch2 produced in Saccharomyces cerevisiae. Molbaek K, Tejada M, ..., Pedersen PA 10.1186/s12934-020-01437-7