Genome location: Pf3D7_14_v3:2,658,255..2,660,911(-)
Genome classification: Core
Product Description: plasmepsin VIII, putative
SignalP Peptide: MNILFCLFVITNLYNIIAVKA
# Transmembrane Domains: 0
EC Numbers: 3.4.17.4 (Gly-Xaa carboxypeptidase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Process | GO:0071976 | cell gliding |
| Process | GO:0035891 | exit from host cell |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.00 | 1.00 |
| Trophozoite | not expressed | 0.01 | 0.99 |
| Schizont | not expressed | 0.00 | 1.00 |
| Gametocyte | expressed | 0.64 | 0.71 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1465700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.946 | MFS: -3.045 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1329100)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1329100)
Modification: Disrupted | RMgm-4128
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKI0
"Best" AlphaFill ligand hit: IVA (isovaleric acid, Local RMSD=0.50) with 3FNT (Global RMSD=3.40)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.17.4 | Gly-Xaa carboxypeptidase | EDTAPMSFCuSO4iodoacetateiodoacetamide1,10-phenanthrolinep-chloromercuribenzoatediisopropylphosphorofluoridatep-chloromercuriphenylsulfonic acid |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q8IM16 | Plasmepsin IV [1-448] | HOGENOM | phenyl-substituted sulfonamides, 6dphenyl-substituted sulfonamides, 6eCHEMBL588662US9353089, 117... | |
| O60989 | Plasmepsin IV, putative | Plasmodium vivax | HOGENOM | CHEMBL575115CHEMBL584189CHEMBL575150CHEMBL584190... |
| P00791 | Pepsin A | Porcellium fiumanum | OMA | CGP 38560phenyl-substituted sulfonamides, 6aphenyl-substituted sulfonamides, 6dphenyl-substituted sulfonamides, 6f... |
Protein Length: 385 | Molecular Weight (kDa): 44.254
UniProt ID(s): Q8IKI0
PDB ID(s): None
Isoelectric Point: 9.37
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001461 | Aspartic peptidase A1 family |
| InterPro | IPR001969 | Aspartic peptidase, active site |
| InterPro | IPR021109 | Aspartic peptidase domain superfamily |
| InterPro | IPR033121 | Peptidase family A1 domain |
| InterPro | IPR034164 | Pepsin-like domain |
| PFam | PF00026 | Peptidase family A1 domain |
| Superfamily | SSF50630 | Aspartic peptidase domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 21 | 14 | 30 |
| disruptive | 4 | 19 | 12 | 43 |
| missense | 4 | 19 | 12 | 39 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 38 | 17 | 35 |
| disruptive | 7 | 52 | 31 | 75 |
| missense | 5 | 42 | 28 | 60 |
PlasmoDB Total SNPs: 107
Non-coding: 83 | Synonymous: 19 | Nonsynonymous: 5 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 28192122 | Plasmodium berghei plasmepsin VIII is essential for sporozoite gliding motility. | Mastan BS, Narwal SK, ..., Mishra S | 10.1016/j.ijpara.2016.11.009 |
| 28351685 | Transcript and protein expression analysis of proteases in the blood stages ofPlasmodium falciparum. | Weissbach T, Golzmann A, ..., Julius Ngwa C | 10.1016/j.exppara.2017.03.006 |