Genome location: Pf3D7_14_v3:2,689,986..2,693,898(+)
Genome classification: Core
Product Description: protein phosphatase containing kelch-like domains
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.3.16 (Protein-serine/threonine phosphatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Component | GO:0008287 | protein serine/threonine phosphatase complex |
| Function | GO:0004722 | protein serine/threonine phosphatase activity |
| Process | GO:0006470 | protein dephosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.19 | 0.87 |
| Schizont | expressed | 0.15 | 0.89 |
| Gametocyte | expressed | 0.11 | 0.93 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1466100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.302 | MFS: -2.61 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1329500)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1329500)
Modification: Disrupted | RMgm-3546
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKH5
"Best" AlphaFill ligand hit: NHE (2-[n-cyclohexylamino]ethane sulfonic acid, Local RMSD=0.25) with 6GHM (Global RMSD=7.78)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.3.16 | protein-serine/threonine phosphatase | ATPADPUDPAMPGTPUTPUMPNaFEDTAEGTAcAMPNADPH... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 17 | 54 | 30 | 55 |
| disruptive | 14 | 50 | 21 | 65 |
| missense | 7 | 41 | 19 | 55 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 29 | 103 | 25 | 39 |
| disruptive | 25 | 94 | 55 | 115 |
| missense | 13 | 77 | 45 | 78 |
PlasmoDB Total SNPs: 121
Non-coding: 70 | Synonymous: 32 | Nonsynonymous: 16 | Stop Codon: 3
Protein Length: 889 | Molecular Weight (kDa): 100.775
PDB ID(s): None
Isoelectric Point: 7.26
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004843 | Calcineurin-like phosphoesterase domain, ApaH type |
| InterPro | IPR006186 | Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase |
| InterPro | IPR006652 | Kelch repeat type 1 |
| InterPro | IPR011043 | Galactose oxidase/kelch, beta-propeller |
| InterPro | IPR011498 | Kelch repeat type 2 |
| InterPro | IPR012391 | Serine/threonine protein phosphatase, BSU1 |
| InterPro | IPR015915 | Kelch-type beta propeller |
| InterPro | IPR041758 | Phosphatase BSL, C-terminal metallophosphatase domain |
| PFam | PF00149 | Calcineurin-like phosphoesterase domain, ApaH type |
| PFam | PF01344 | Kelch repeat type 1 |
| PFam | PF07646 | Kelch repeat type 2 |
| PFam | PF13418 | |
| Superfamily | SSF117281 | Kelch-type beta propeller |
| Superfamily | SSF50965 | Galactose oxidase/kelch, beta-propeller |
| Superfamily | SSF56300 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9803420 | A putative protein serine/threonine phosphatase from Plasmodium falciparumcontains a large N-terminal extension and five unique inserts in the catalyticdomain. | Li JL, Baker DA | 10.1016/s0166-6851(98)00106-6 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 22957089 | A unique Kelch domain phosphatase in Plasmodium regulates ookinete morphology,motility and invasion. | Philip N, Vaikkinen HJ, Tetley L, Waters AP | 10.1371/journal.pone.0044617 |
| 23028336 | A unique protein phosphatase with kelch-like domains (PPKL) in Plasmodiummodulates ookinete differentiation, motility and invasion. | Guttery DS, Poulin B, ..., Tewari R | 10.1371/journal.ppat.1002948 |
| 37398039 | The protein phosphatase PPKL is a key regulator of daughter parasite development in Toxoplasma gondii | Yang C, Doud EH, Sampson E, Arrizabalaga G | 10.1101/2023.06.13.544803 |
| 37877735 | The protein phosphatase PPKL is a key regulator of daughter parasite developmentin Toxoplasma gondii | Yang C, Doud EH, Sampson E, Arrizabalaga G | 10.1128/mbio.02254-23 |
| 24492333 | Revisiting the evolutionary history and roles of protein phosphatases with Kelch-like domains in plants | Maselli GA, Slamovits CH, ..., Mora-Garcia S | 10.1104/pp.113.233627 |
| 12034355 | Protein Ser/Thr phosphatases with kelch-like repeat domains | Kutuzov MA, Andreeva AV | 10.1016/s0898-6568(02)00018-9 |