Genome location: Pf3D7_14_v3:2,709,036..2,713,626(+)
Genome classification: Core
Product Description: 26S proteasome regulatory subunit RPN2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.25.1 (Proteasome endopeptidase complex)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Component | GO:0005838 | proteasome regulatory particle |
| Component | GO:0008540 | proteasome regulatory particle, base subcomplex |
| Component | GO:0034515 | proteasome storage granule |
| Function | GO:0004175 | endopeptidase activity |
| Process | GO:0043161 | proteasome-mediated ubiquitin-dependent protein catabolic process |
| Process | GO:0006511 | ubiquitin-dependent protein catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.49 | 0.76 |
| Trophozoite | expressed | 1.18 | 0.42 |
| Schizont | expressed | 1.14 | 0.44 |
| Gametocyte | expressed | 0.71 | 0.62 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1466300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.3 | MFS: -2.601 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1329700)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKH3
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.25.1 | proteasome endopeptidase complex | ATPSDSPSIEDTANH4+EGTATLCKALLNNMDANLVSPI 1PA28... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101410
Most Similar Human Ortholog: F8WCE3
TM-align score: 0.86 | RMSD: 1.28
Seq Identity: 0.48 | Length: 48 / 1172
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000173692 | proteasome 26S subunit, non-ATPase 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 71 | 43 | 76 |
| disruptive | 14 | 101 | 49 | 153 |
| missense | 14 | 92 | 43 | 117 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 151 | 57 | 85 |
| disruptive | 33 | 216 | 112 | 285 |
| missense | 28 | 179 | 92 | 149 |
PlasmoDB Total SNPs: 114
Non-coding: 59 | Synonymous: 32 | Nonsynonymous: 23 | Stop Codon: 0
Protein Length: 1172 | Molecular Weight (kDa): 132.767
UniProt ID(s): Q8IKH3
PDB ID(s): None
Isoelectric Point: 4.9
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002015 | Proteasome/cyclosome repeat |
| InterPro | IPR004155 | PBS lyase HEAT-like repeat |
| InterPro | IPR016024 | Armadillo-type fold |
| InterPro | IPR016642 | 26S proteasome regulatory complex, non-ATPase subcomplex, Rpn2/Psmd1 subunit |
| InterPro | IPR035266 | 26S Proteasome non-ATPase regulatory subunit 1 |
| InterPro | IPR040623 | 26S proteasome regulatory subunit RPN2, C-terminal |
| PFam | PF01851 | Proteasome/cyclosome repeat |
| PFam | PF13646 | 26S proteasome regulatory subunit RPN2, C-terminal |
| PFam | PF18004 | |
| Superfamily | SSF48371 | Armadillo-type fold |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 38404287 | Mutation in the 26S proteasome regulatory subunit rpn2 gene in Plasmodium falciparum confers resistance to artemisinin | Goncalves AF, Lima-Pinheiro A, ..., Ferreira PE | 10.3389/fcimb.2024.1342856 |
| 34778038 | Knockdown of Oligosaccharyltransferase Subunit Ribophorin 1 Induces Endoplasmic-Reticulum-Stress-Dependent Cell Apoptosis in Breast Cancer | Ding J, Xu J, ..., Zhang L | 10.3389/fonc.2021.722624 |