Genome location: Pf3D7_14_v3:2,744,003..2,747,949(-)
Genome classification: Core
Product Description: DNA-3-methyladenine glycosylase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.2.2.21 (DNA-3-methyladenine glycosylase II)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0019104 | DNA N-glycosylase activity |
| Function | GO:0003905 | alkylbase DNA N-glycosylase activity |
| Process | GO:0006284 | base-excision repair |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.11 | 0.95 |
| Trophozoite | expressed | 0.24 | 0.85 |
| Schizont | expressed | 0.05 | 0.97 |
| Gametocyte | not expressed | 0.10 | 0.94 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1467100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.12 | #Insertions: 3
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1330400)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1330400)
Modification: Disrupted | RMgm-3550
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKG6
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.2.2.21 | DNA-3-methyladenine glycosylase II | CaCl2ZnSO4apurinic-DNAN-ethylmaleimidep-mercuribenzoateDouble-stranded DNAabasic-containing DNAp-hydroxymercuribenzoateDNA containing 1-azaribose2'-deoxy-2'-fluoro-7-methylguanosinePyrrolidine-containing oligonucleotide |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P29372 | DNA-3-methyladenine glycosylase | OrthoDB, OrthoMCL BLAST | MLS000069618Gossypol (C9)MITOXANTRONEAurintricarboxylic acid... |
Ortholog Group (OrthoMCL): OG6_104748
Most Similar Human Ortholog: A2IDA3
TM-align score: 0.70 | RMSD: 2.80
Seq Identity: 0.26 | Length: 195 / 501
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000103152 | N-methylpurine DNA glycosylase |
Protein Length: 501 | Molecular Weight (kDa): 59.37
UniProt ID(s): Q8IKG6
PDB ID(s): None
Isoelectric Point: 9.32
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003180 | Methylpurine-DNA glycosylase |
| InterPro | IPR011034 | Formyl transferase-like, C-terminal domain superfamily |
| PFam | PF02245 | Methylpurine-DNA glycosylase |
| Superfamily | SSF50486 | Formyl transferase-like, C-terminal domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 34 | 14 | 40 |
| disruptive | 13 | 78 | 46 | 112 |
| missense | 8 | 70 | 44 | 97 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 65 | 20 | 39 |
| disruptive | 29 | 155 | 86 | 158 |
| missense | 18 | 133 | 80 | 108 |
PlasmoDB Total SNPs: 117
Non-coding: 69 | Synonymous: 26 | Nonsynonymous: 22 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 32762689 | Molecular characterization of Plasmodium falciparum DNA-3-methyladenineglycosylase. | Pinthong N, Limudomporn P, ..., Chavalitshewinkoon-Petmitr P | 10.1186/s12936-020-03355-w |