Genome location: Pf3D7_14_v3:2,827,067..2,830,703(-)
Genome classification: Core
Product Description: splicing factor U2AF large subunit, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0071004 | U2-type prespliceosome |
| Component | GO:0089701 | U2AF complex |
| Component | GO:0000243 | commitment complex |
| Component | GO:0016607 | nuclear speck |
| Component | GO:0005634 | nucleus |
| Component | GO:0005681 | spliceosomal complex |
| Function | GO:0003723 | RNA binding |
| Function | GO:0008187 | poly-pyrimidine tract binding |
| Function | GO:0030628 | pre-mRNA 3'-splice site binding |
| Process | GO:0000398 | mRNA splicing, via spliceosome |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.32 | 0.85 |
| Trophozoite | expressed | 0.48 | 0.73 |
| Schizont | expressed | 0.13 | 0.91 |
| Gametocyte | expressed | 0.66 | 0.64 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1468800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.154 | MFS: -2.78 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1332000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKE9
"Best" AlphaFill ligand hit: 1PG (2-(2-{2-[2-(2-methoxy-ethoxy)-ethoxy]-ethoxy}-ethoxy)-ethanol, Local RMSD=0.48) with 5W0G (Global RMSD=3.33)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101356
Most Similar Human Ortholog: P26368
TM-align score: 0.38 | RMSD: 5.58
Seq Identity: 0.16 | Length: 235 / 833
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000063244 | U2 small nuclear RNA auxiliary factor 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 48 | 16 | 59 |
| disruptive | 16 | 138 | 74 | 151 |
| missense | 14 | 126 | 68 | 134 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 99 | 39 | 51 |
| disruptive | 55 | 248 | 90 | 182 |
| missense | 36 | 220 | 84 | 143 |
PlasmoDB Total SNPs: 171
Non-coding: 119 | Synonymous: 25 | Nonsynonymous: 27 | Stop Codon: 0
Protein Length: 833 | Molecular Weight (kDa): 97.428
UniProt ID(s): Q8IKE9
PDB ID(s): None
Isoelectric Point: 10.12
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000504 | RNA recognition motif domain |
| InterPro | IPR006529 | U2 snRNP auxilliary factor, large subunit, splicing factor |
| InterPro | IPR035979 | RNA-binding domain superfamily |
| PFam | PF00076 | RNA recognition motif domain |
| Superfamily | SSF54928 | RNA-binding domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |