Genome location: Pf3D7_14_v3:2,838,446..2,840,316(-)
Genome classification: Core
Product Description: shewanella-like protein phosphatase 1, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.3.16 (Protein-serine/threonine phosphatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0005634 | nucleus |
| Function | GO:0016791 | phosphatase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.05 | 0.97 |
| Trophozoite | expressed | 0.09 | 0.94 |
| Schizont | expressed | 0.56 | 0.71 |
| Gametocyte | expressed | 0.21 | 0.87 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1469200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.162 | MFS: -3.303 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0604700)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1332400)
Modification: Tagged | RMgm-1073
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKE5
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.3.16 | protein-serine/threonine phosphatase | ATPADPUDPAMPGTPUTPUMPNaFEDTAEGTAcAMPNADPH... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 22 | 13 | 18 |
| disruptive | 8 | 17 | 11 | 50 |
| missense | 8 | 16 | 11 | 41 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 37 | 17 | 21 |
| disruptive | 13 | 59 | 32 | 99 |
| missense | 9 | 48 | 21 | 61 |
PlasmoDB Total SNPs: 70
Non-coding: 59 | Synonymous: 5 | Nonsynonymous: 5 | Stop Codon: 1
Protein Length: 358 | Molecular Weight (kDa): 42.326
UniProt ID(s): Q8IKE5
PDB ID(s): None
Isoelectric Point: 10.01
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004843 | Calcineurin-like phosphoesterase domain, ApaH type |
| InterPro | IPR041787 | Shewanella-like phosphatase, metallophosphatase domain |
| PFam | PF00149 | Calcineurin-like phosphoesterase domain, ApaH type |
| Superfamily | SSF56300 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23434509 | An ancient protein phosphatase, SHLP1, is critical to microneme development inPlasmodium ookinetes and parasite transmission. | Patzewitz EM, Guttery DS, ..., Tewari R | 10.1016/j.celrep.2013.01.032 |
| 25011111 | Genome-wide functional analysis of Plasmodium protein phosphatases reveals keyregulators of parasite development and differentiation. | Guttery DS, Poulin B, ..., Tewari R | 10.1016/j.chom.2014.05.020 |
| 29073264 | Shelph2, a bacterial-like phosphatase of the malaria parasite Plasmodiumfalciparum, is dispensable during asexual blood stage. | Miliu A, Lebrun M, Braun-Breton C, Lamarque MH | 10.1371/journal.pone.0187073 |
| 31555028 | Homology Modeling and Docking Study of Shewanella-like Protein PhosphataseInvolved in the Development of Ookinetes in Plasmodium | Singh S, Yadav R | 10.4103/jpbs.JPBS_205_18 |