PF3D7_1469300 (PNO1)

Genome location: Pf3D7_14_v3:2,840,696..2,842,391(-)

Genome classification: Core

Function and Localization

Product Description: pre-rRNA-processing protein PNO1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005730	nucleolus
Function	GO:0051082	unfolded protein binding

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.92	0.58
Trophozoite	expressed	0.90	0.53
Schizont	not expressed	0.17	0.90
Gametocyte	expressed	0.68	0.63

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1469300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.132 | MFS: -3.204 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IKE4

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101323

Most Similar Human Ortholog: Q9NRX1

TM-align score: 0.72 | RMSD: 1.30

Seq Identity: 0.51 | Length: 187 / 262

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000115946	partner of NOB1 homolog

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	16	4	11
disruptive	4	19	16	32
missense	4	19	16	31

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	3	23	8	19
disruptive	4	50	28	60
missense	4	46	27	51

PlasmoDB Total SNPs: 66

Non-coding: 52 | Synonymous: 11 | Nonsynonymous: 3 | Stop Codon: 0

Protein Information

Protein Length: 262 | Molecular Weight (kDa): 30.138

UniProt ID(s): Q8IKE4

PDB ID(s): None

Isoelectric Point: 10.6

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004087	K Homology domain
InterPro	IPR004088	K Homology domain, type 1
InterPro	IPR036612	K Homology domain, type 1 superfamily
InterPro	IPR039912	Pre-rRNA-processing protein PNO1-like
PFam	PF00013	K Homology domain, type 1
Superfamily	SSF54791	K Homology domain, type 1 superfamily