Genome location: Pf3D7_14_v3:2,863,545..2,865,220(+)
Genome classification: Core
Product Description: mediator of RNA polymerase II transcription subunit 6, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0016592 | mediator complex |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | not expressed | 0.04 | 0.98 |
| Trophozoite | expressed | 0.41 | 0.75 |
| Schizont | possibly expressed | 0.11 | 0.92 |
| Gametocyte | expressed | 0.40 | 0.76 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1469700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -3.098 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1332900)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKE0
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102673
Most Similar Human Ortholog: G3V4A5
TM-align score: 0.65 | RMSD: 3.06
Seq Identity: 0.20 | Length: 154 / 205
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000133997 | mediator complex subunit 6 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 146
Non-coding: 128 | Synonymous: 13 | Nonsynonymous: 5 | Stop Codon: 0
Protein Length: 205 | Molecular Weight (kDa): 24.568
UniProt ID(s): Q8IKE0
PDB ID(s): None
Isoelectric Point: 9.07
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR007018 | Mediator complex, subunit Med6 |
| PFam | PF04934 | Mediator complex, subunit Med6 |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 35557691 | Mediator Complex of the Malaria Parasite Plasmodium falciparum Associates withEvolutionarily Novel Subunits. | Iyer UB, Park JE, ..., Featherstone M | 10.1021/acsomega.2c00368 |
| 37014863 | Yeast PIC-Mediator structure with RNA polymerase II C-terminal domain | Schilbach S, Wang H, Dienemann C, Cramer P | 10.1073/pnas.2220542120 |
| 34627408 | Genetic interaction between RLM1 and F-box motif encoding gene SAF1 contributesto stress response in Saccharomyces cerevisiae | Sharma M, Verma V, Bairwa NK | 10.1186/s41021-021-00218-x |
| 38045341 | A Potent Kalihinol Analogue Disrupts Apicoplast Function and VesicularTrafficking in P. falciparum Malaria | Chahine Z, Abel S, ..., Le Roch KG | 10.1101/2023.11.21.568162 |
| 29992056 | Postrecruitment Function of Yeast Med6 Protein during the TranscriptionalActivation by Mediator Complex | Kim GS, Lee YC | 10.1155/2018/6406372 |
| 27317677 | Promotion of Cell Viability and Histone Gene Expression by the AcetyltransferaseGcn5 and the Protein Phosphatase PP2A in Saccharomyces cerevisiae | Petty EL, Lafon A, ..., Pillus L | 10.1534/genetics.116.189506 |