Genome location: Pf3D7_14_v3:2,901,717..2,903,840(+)
Genome classification: Core
Product Description: RNA 3'-terminal phosphate cyclase-like protein, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.5.1.4 (RNA 3'-terminal-phosphate cyclase (ATP))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0030686 | 90S preribosome |
| Component | GO:0005730 | nucleolus |
| Function | GO:0003963 | RNA-3'-phosphate cyclase activity |
| Function | GO:0004521 | endoribonuclease activity |
| Process | GO:0000479 | endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) |
| Process | GO:0006364 | rRNA processing |
| Process | GO:0042254 | ribosome biogenesis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.16 | 0.91 |
| Trophozoite | expressed | 0.12 | 0.92 |
| Schizont | expressed | 0.01 | 0.99 |
| Gametocyte | expressed | 0.11 | 0.93 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1471000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.121 | MFS: -3.129 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I717
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.5.1.4 | RNA 3'-terminal-phosphate cyclase (ATP) | NEMSDSpC3'piodoacetamideSodium phosphateVanadyl-ribonucleoside complex |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102522
Most Similar Human Ortholog: Q9Y2P8
TM-align score: 0.92 | RMSD: 1.89
Seq Identity: 0.32 | Length: 361 / 467
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000120158 | RNA terminal phosphate cyclase like 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 37 | 18 | 44 |
| disruptive | 4 | 57 | 43 | 72 |
| missense | 4 | 56 | 43 | 71 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 65 | 20 | 53 |
| disruptive | 14 | 135 | 75 | 133 |
| missense | 12 | 118 | 65 | 89 |
PlasmoDB Total SNPs: 136
Non-coding: 99 | Synonymous: 27 | Nonsynonymous: 10 | Stop Codon: 0
Protein Length: 467 | Molecular Weight (kDa): 54.895
UniProt ID(s): Q8I717
PDB ID(s): None
Isoelectric Point: 8.85
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000228 | RNA 3'-terminal phosphate cyclase |
| InterPro | IPR013791 | RNA 3'-terminal phosphate cyclase, insert domain |
| InterPro | IPR013792 | RNA 3'-terminal phosphate cyclase/enolpyruvate transferase, alpha/beta |
| InterPro | IPR016443 | RNA 3'-terminal phosphate cyclase type 2 |
| InterPro | IPR023797 | RNA 3'-terminal phosphate cyclase domain |
| PFam | PF01137 | RNA 3'-terminal phosphate cyclase domain |
| PFam | PF05189 | RNA 3'-terminal phosphate cyclase, insert domain |
| Superfamily | SSF55205 | RNA 3'-terminal phosphate cyclase/enolpyruvate transferase, alpha/beta |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 37451810 | Stability and function of RCL1 are dependent on interaction with BMS1 | Wang Y, Zhao Z, ..., Lo LJ | 10.1093/jmcb/mjad046 |