Genome location: Pf3D7_14_v3:2,903,947..2,907,414(-)
Genome classification: Core
Product Description: exported protein 2
SignalP Peptide: MKVSYIFSFFLLFFVYKNTNT
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0097619 | PTEX complex |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0044161 | host cell cytoplasmic vesicle |
| Component | GO:0020026 | merozoite dense granule |
| Component | GO:0005634 | nucleus |
| Component | GO:0020005 | symbiont-containing vacuole membrane |
| Function | GO:0003723 | RNA binding |
| Function | GO:0015267 | channel activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0044409 | entry into host |
| Process | GO:0044658 | pore formation in membrane of host by symbiont |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.62 | 0.38 |
| Trophozoite | expressed | 2.67 | 0.12 |
| Schizont | expressed | 0.41 | 0.78 |
| Gametocyte | expressed | 0.60 | 0.71 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1471100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -3.183 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1334300)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1334300)
Modification: Mutated | RMgm-5258
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKC8
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 26 | 8 | 24 |
| disruptive | 10 | 14 | 8 | 27 |
| missense | 5 | 11 | 5 | 17 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 16 | 41 | 19 | 22 |
| disruptive | 15 | 40 | 31 | 72 |
| missense | 7 | 30 | 20 | 42 |
PlasmoDB Total SNPs: 271
Non-coding: 251 | Synonymous: 15 | Nonsynonymous: 5 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 22253438 | Biosynthesis, localization, and macromolecular arrangement of the Plasmodiumfalciparum translocon of exported proteins (PTEX). | Bullen HE, Charnaud SC, ..., Gilson PR | 10.1074/jbc.M111.328591 |
| 25043010 | PTEX component HSP101 mediates export of diverse malaria effectors into hosterythrocytes. | Beck JR, Muralidharan V, Oksman A, Goldberg DE | 10.1038/nature13574 |
| 25662767 | Host erythrocyte environment influences the localization of exported protein 2,an essential component of the Plasmodium translocon. | Meibalan E, Comunale MA, ..., Burns JM Jr | 10.1128/EC.00228-14 |
| 27168322 | Stable Translocation Intermediates Jam Global Protein Export in Plasmodiumfalciparum Parasites and Link the PTEX Component EXP2 with TranslocationActivity. | Mesen-Ramirez P, Reinsch F, ..., Spielmann T | 10.1371/journal.ppat.1005618 |
| 27865834 | Expression and characterization of the Plasmodium translocon of the exportedproteins component EXP2. | Hakamada K, Watanabe H, ..., Yohda M | 10.1016/j.bbrc.2016.11.097 |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 29487358 | PV1, a novel Plasmodium falciparum merozoite dense granule protein, interactswith exported protein in infected erythrocytes. | Morita M, Nagaoka H, ..., Takashima E | 10.1038/s41598-018-22026-0 |
| 30150733 | EXP2 is a nutrient-permeable channel in the vacuolar membrane of Plasmodium andis essential for protein export via PTEX. | Garten M, Nasamu AS, ..., Beck JR | 10.1038/s41564-018-0222-7 |
| 30150771 | Malaria parasite translocon structure and mechanism of effector export. | Ho CM, Beck JR, ..., Zhou ZH | 10.1038/s41586-018-0469-4 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 30439948 | Knockdown of the translocon protein EXP2 in Plasmodium falciparum reduces growthand protein export. | Charnaud SC, Kumarasingha R, ..., Gilson PR | 10.1371/journal.pone.0204785 |
| 30476118 | The N-terminus of EXP2 forms the membrane-associated pore of the proteinexporting translocon PTEX in Plasmodium falciparum. | Sanders PR, Dickerman BK, ..., Gilson PR | 10.1093/jb/mvy099 |
| 31568532 | EXP1 is critical for nutrient uptake across the parasitophorous vacuole membraneof malaria parasites. | Mesen-Ramirez P, Bergmann B, ..., Spielmann T | 10.1371/journal.pbio.3000473 |
| 33159090 | Plasmodium translocon component EXP2 facilitates hepatocyte invasion. | Mello-Vieira J, Enguita FJ, ..., Mota MM | 10.1038/s41467-020-19492-4 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 35192672 | A revised mechanism for how Plasmodium falciparum recruits and exports proteinsinto its erythrocytic host cell. | Gabriela M, Matthews KM, ..., Gilson PR | 10.1371/journal.ppat.1009977 |
| 38282616 | Dissecting EXP2 sequence requirements for protein export in malaria parasites | Pitman EL, Counihan NA, ..., de Koning-Ward TF | 10.3389/fcimb.2023.1332146 |
| 37552754 | Knock-sideways by inducible ER retrieval enables a unique approach for studyingPlasmodium-secreted proteins | Fierro MA, Hussain T, Campin LJ, Beck JR | 10.1073/pnas.2308676120 |
| 37939134 | NK cell-induced damage to P.falciparum-infected erythrocytes requiresligand-specific recognition and releases parasitophorous vacuoles that arephagocytosed by monocytes in the presence of immune IgG | Sekar P, Rajagopalan S, ..., Long EO | 10.1371/journal.ppat.1011585 |
| 36126089 | A member of the tryptophan-rich protein family is required for efficientsequestration of Plasmodium berghei schizonts | Gabelich JA, Grutzke J, ..., Ingmundson A | 10.1371/journal.ppat.1010846 |
| 36445080 | The PTEX Pore Component EXP2 Is Important for Intrahepatic Development during thePlasmodium Liver Stage | Hussain T, Linera-Gonzalez J, ..., Beck JR | 10.1128/mbio.03096-22 |