PF3D7_1471400

Genome location: Pf3D7_14_v3:2,918,191..2,921,490(-)

Genome classification: Core

Function and Localization

Product Description: diacylglycerol kinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.1.107 (Diacylglycerol kinase (ATP))

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Function	GO:0004143	diacylglycerol kinase activity
Process	GO:0007205	protein kinase C-activating G protein-coupled receptor signaling pathway
Process	GO:0019932	second-messenger-mediated signaling

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.05	0.97
Trophozoite	expressed	0.36	0.78
Schizont	expressed	0.77	0.55
Gametocyte	expressed	0.37	0.78

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1471400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.129 | MFS: -2.881 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1334600)

Relative Growth Rate: 0.37 ± 0.12
Confidence: 5.71

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1334600)

Modification: Disrupted | RMgm-3565

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IKC5

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.1.107	diacylglycerol kinase (ATP)	ATPADPUDPAMPGTPGDPCTPUTPCDPITPIDPcAMP...

Orthology to BindingDB Entries:

UniProt	Protein Name	Homology Source	BindingDB Ligands
P23743	Diacylglycerol kinase alpha/zeta	OrthoDB	US11584747, Example 2US11584747, Example 2US11584747, Example 4US11584747, Example 4...
Q13574	Diacylglycerol kinase zeta	OrthoDB	US11584747, Example 2US11584747, Example 2US11584747, Example 4US11584747, Example 4...
P20192	Diacylglycerol kinase alpha	OrthoDB	CHEMBL4558937CHEMBL4558937

Orthology Information

Ortholog Group (OrthoMCL): OG6_117937

No human ortholog(s)

Protein Information

Protein Length: 499 | Molecular Weight (kDa): 57.642

UniProt ID(s): Q8IKC5

PDB ID(s): None

Isoelectric Point: 9.18

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000756	Diacylglycerol kinase, accessory domain
InterPro	IPR001206	Diacylglycerol kinase, catalytic domain
InterPro	IPR016064	NAD kinase/diacylglycerol kinase-like domain superfamily
InterPro	IPR037607	Diacylglycerol kinase
PFam	PF00609	Diacylglycerol kinase, accessory domain
PFam	PF00781	Diacylglycerol kinase, catalytic domain
Superfamily	SSF111331	NAD kinase/diacylglycerol kinase-like domain superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	9	25	22	25
disruptive	17	53	27	70
missense	12	39	24	52

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	14	59	14	34
disruptive	39	113	62	146
missense	25	82	46	86

PlasmoDB Total SNPs: 238

Non-coding: 189 | Synonymous: 25 | Nonsynonymous: 24 | Stop Codon: 0

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108