Genome location: Pf3D7_14_v3:2,940,178..2,941,916(+)
Genome classification: Core
Product Description: pre-mRNA-splicing factor ISY1, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0000974 | Prp19 complex |
| Component | GO:0071013 | catalytic step 2 spliceosome |
| Component | GO:0071014 | post-mRNA release spliceosomal complex |
| Component | GO:0071020 | post-spliceosomal complex |
| Process | GO:0000350 | generation of catalytic spliceosome for second transesterification step |
| Process | GO:0000389 | mRNA 3'-splice site recognition |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.16 | 0.92 |
| Trophozoite | expressed | 0.15 | 0.91 |
| Schizont | possibly expressed | 0.02 | 0.98 |
| Gametocyte | possibly expressed | 0.57 | 0.68 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1472000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.138 | MFS: -3.186 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKB8
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102669
Most Similar Human Ortholog: D6RC18
TM-align score: 0.83 | RMSD: 2.10
Seq Identity: 0.39 | Length: 117 / 201
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000240682 | ISY1 splicing factor homolog |
| ENSG00000261796 | ISY1-RAB43 readthrough |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 12 | 11 | 20 |
| disruptive | 1 | 9 | 11 | 30 |
| missense | 1 | 9 | 11 | 25 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 27 | 11 | 19 |
| disruptive | 2 | 39 | 25 | 58 |
| missense | 1 | 28 | 24 | 33 |
PlasmoDB Total SNPs: 146
Non-coding: 141 | Synonymous: 4 | Nonsynonymous: 1 | Stop Codon: 0
Protein Length: 201 | Molecular Weight (kDa): 24
UniProt ID(s): Q8IKB8
PDB ID(s): None
Isoelectric Point: 10.11
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR009360 | Pre-mRNA-splicing factor Isy1 |
| InterPro | IPR037200 | Pre-mRNA-splicing factor Isy1 superfamily |
| PFam | PF06246 | Pre-mRNA-splicing factor Isy1 |
| Superfamily | SSF140102 | Pre-mRNA-splicing factor Isy1 superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 19789211 | A systematic characterization of Cwc21, the yeast ortholog of the humanspliceosomal protein SRm300 | Khanna M, Van Bakel H, ..., Blencowe BJ | 10.1261/rna.1790509 |
| 34850936 | A distinct complex of PRP19-related and trypanosomatid-specific proteins isrequired for pre-mRNA splicing in trypanosomes | Srivastava A, Ambrosio DL, ..., Gunzl A | 10.1093/nar/gkab1152 |