PF3D7_1472400

Genome location: Pf3D7_14_v3:2,957,291..2,961,663(+)

Genome classification: Core

Function and Localization

Product Description: M1-family alanyl aminopeptidase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 5

EC Numbers: 3.4.11.2 (Membrane alanyl aminopeptidase)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.27	0.86
Trophozoite	not expressed	0.43	0.75
Schizont	not expressed	0.31	0.80
Gametocyte	not expressed	0.31	0.83

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1472400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.621 | #Insertions: 5

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1335600)

Relative Growth Rate: 0.94 ± 0.16
Confidence: 5.07

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1335600)

Modification: Disrupted | RMgm-3569

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IKB4

"Best" AlphaFill ligand hit: 1OT ({(r)-amino[4-(1h-pyrazol-1-yl)phenyl]methyl}phosphonic acid, Local RMSD=1.71) with 4K5O (Global RMSD=8.41)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.11.2	membrane alanyl aminopeptidase	NEMKCNLeuProE64EDTAEGTAPMSFSCN-PC18EC27L-Arg...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532703

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	13	89	40	114
disruptive	56	285	153	300
missense	46	249	136	255

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	26	166	79	109
disruptive	128	567	209	448
missense	88	476	167	302

PlasmoDB Total SNPs: 206

Non-coding: 67 | Synonymous: 74 | Nonsynonymous: 65 | Stop Codon: 0

Protein Information

Protein Length: 1281 | Molecular Weight (kDa): 156.158

UniProt ID(s): Q8IKB4

PDB ID(s): None

Isoelectric Point: 10.39

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR014782	Peptidase M1, membrane alanine aminopeptidase
PFam	PF01433	Peptidase M1, membrane alanine aminopeptidase
Superfamily	SSF55486	N/A