PF3D7_1472600 (PDI-14)

Genome location: Pf3D7_14_v3:2,962,911..2,965,133(-)

Genome classification: Core

Function and Localization

Product Description: protein disulfide-isomerase

SignalP Peptide: MHIDKLLIVFIFLIKLCVC

# Transmembrane Domains: 0

EC Numbers: 5.3.4.1 (Protein disulfide-isomerase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005783	endoplasmic reticulum
Function	GO:0003756	protein disulfide isomerase activity
Process	GO:0006457	protein folding
Process	GO:0034976	response to endoplasmic reticulum stress

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.04	0.98
Trophozoite	expressed	0.14	0.91
Schizont	expressed	0.88	0.54
Gametocyte	expressed	0.26	0.84

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1472600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.63 | #Insertions: 3

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1335800)

Relative Growth Rate: 0.99 ± 0.10
Confidence: 6.06

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1335800)

Modification: Disrupted | RMgm-3570

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IKB2

"Best" AlphaFill ligand hit: FAD (flavin-adenine dinucleotide, Local RMSD=0.39) with 4P2L (Global RMSD=4.05)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
5.3.4.1	protein disulfide-isomerase	NEM16F16LOC14AsO43-CCF642MA3 018MA3 019acroleinneomycinpeptidesgenisteinkanamycin...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P30101	Protein disulfide-isomerase A3		OrthoDB	CHEMBL4751979
P07237	Protein disulfide-isomerase	Homo sapiens	OrthoDB	Juniferdin derivative, 1Juniferdin derivative, 3Juniferdin derivative, 4Juniferdin derivative, 5...
P05307	Protein disulfide-isomerase		OrthoDB	16F16A

Orthology Information

Ortholog Group (OrthoMCL): OG6_133230

No human ortholog(s)

Protein Information

Protein Length: 553 | Molecular Weight (kDa): 66.518

UniProt ID(s): Q8IKB2

PDB ID(s): None

Isoelectric Point: 5.45

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR013766	Thioredoxin domain
InterPro	IPR017937	Thioredoxin, conserved site
InterPro	IPR036249	Thioredoxin-like superfamily
PFam	PF00085	Thioredoxin domain
Superfamily	SSF52833	Thioredoxin-like superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	34	20	41
disruptive	13	92	50	113
missense	13	91	47	109

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	12	60	31	35
disruptive	30	184	72	127
missense	30	169	66	99

PlasmoDB Total SNPs: 145

Non-coding: 100 | Synonymous: 25 | Nonsynonymous: 20 | Stop Codon: 0