About Gene List

PF3D7_1472700

Genome location: Pf3D7_14_v3:2,966,676..2,969,387(+)

Genome classification: Core

Function and Localization

Product Description: DNA-directed RNA polymerase, alpha subunit, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020011 apicoplast
Component GO:0005737 cytoplasm
Function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
Process GO:0006351 transcription, DNA-templated

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring not expressed 0.09 0.95
Trophozoite expressed 0.55 0.68
Schizont expressed 0.59 0.66
Gametocyte expressed 0.85 0.57

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1472700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 5 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.168 | MFS: -2.71 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IKB1

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
2.7.7.6 DNA-directed RNA polymerase BRENDA SOAP query unsuccessful

Orthology to BindingDB Entries:

UniProt Protein Name Species Homology Source BindingDB Ligands
P0A7Z4 DNA-directed RNA polymerase subunit alpha OrthoMCL, OrthoMCL BLAST US10800725, Compound 15US10800725, Compound 19US10800725, Compound 1US10800725, Compound 2...
P9WGZ1 DNA-directed RNA polymerase subunit alpha OrthoMCL, OrthoMCL BLAST US10800725, Compound 15US10800725, Compound 19US10800725, Compound 20US10800725, Compound 1...
P66706 DNA-directed RNA polymerase subunit alpha OrthoMCL BLAST US10800725, Compound 1US10800725, Compound 2US10800725, Compound 3US10800725, Compound 4...

Orthology Information

Ortholog Group (OrthoMCL): OG6_105781

No human ortholog(s)

Protein Information

Protein Length: 861 | Molecular Weight (kDa): 101.938

UniProt ID(s): Q8IKB1

PDB ID(s): None

Isoelectric Point: 9.02

Protein Domain Annotations:

Source Family ID Description
InterPro IPR011263 DNA-directed RNA polymerase, RpoA/D/Rpb3-type
InterPro IPR036603 RNA polymerase, RBP11-like subunit
InterPro IPR036643 DNA-directed RNA polymerase, insert domain superfamily
PFam PF01193 DNA-directed RNA polymerase, RpoA/D/Rpb3-type
Superfamily SSF55257 RNA polymerase, RBP11-like subunit
Superfamily SSF56553 DNA-directed RNA polymerase, insert domain superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 10 41 24 51
disruptive 27 89 37 140
missense 18 61 25 110

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 14 83 41 65
disruptive 59 203 86 229
missense 38 139 58 153

PlasmoDB Total SNPs: 116

Non-coding: 10 | Synonymous: 30 | Nonsynonymous: 76 | Stop Codon: 0

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
30212465 Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. Boucher MJ, Ghosh S, ..., Yeh E 10.1371/journal.pbio.2005895