About Gene List

PF3D7_1472900

Genome location: Pf3D7_14_v3:2,972,727..2,975,092(+)

Genome classification: Core

Function and Localization

Product Description: dihydroorotase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.5.2.3 (Dihydroorotase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005737 cytoplasm
Component GO:0005634 nucleus
Function GO:0004151 dihydroorotase activity
Process GO:0006207 'de novo' pyrimidine nucleobase biosynthetic process
Process GO:0006221 pyrimidine nucleotide biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring expressed 0.06 0.97
Trophozoite expressed 0.12 0.93
Schizont expressed 0.04 0.98
Gametocyte expressed 0.69 0.65

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1472900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.259 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IKA9

"Best" AlphaFill ligand hit: ORO (orotic acid, Local RMSD=0.30) with 1J79 (Global RMSD=4.32)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
3.5.2.3 dihydroorotase BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_106265

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 3 29 8 26
disruptive 2 7 8 22
missense 2 7 8 18

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 10 53 11 26
disruptive 5 28 26 70
missense 3 17 24 52

PlasmoDB Total SNPs: 121

Non-coding: 104 | Synonymous: 13 | Nonsynonymous: 4 | Stop Codon: 0

Protein Information

Protein Length: 358 | Molecular Weight (kDa): 41.718

UniProt ID(s): A9CSR1, Q8IKA9

PDB ID(s): None

Isoelectric Point: 7.38

Protein Domain Annotations:

Source Family ID Description
InterPro IPR002195 Dihydroorotase, conserved site
InterPro IPR004721 Dihydroorotase homodimeric type
InterPro IPR032466 Metal-dependent hydrolase
PFam N/A N/A
Superfamily SSF51556 Metal-dependent hydrolase

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
15571277 Cloning and expression of malarial pyrimidine enzymes. Christopherson RI, Cinquin O, ..., Menz RI 10.1081/NCN-200027678
23181666 Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. Oehring SC, Woodcroft BJ, ..., Voss TS 10.1186/gb-2012-13-11-r108
30379851 Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. Briquet S, Ourimi A, ..., Vaquero C 10.1371/journal.pone.0205596