PF3D7_1474300

Genome location: Pf3D7_14_v3:3,040,666..3,043,907(+)

Genome classification: Core

Function and Localization

Product Description: DNA repair metallo-beta-lactamase protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.5.2.6 (Beta-lactamase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0035312	5'-3' exodeoxyribonuclease activity
Function	GO:0003684	damaged DNA binding
Process	GO:0006303	double-strand break repair via nonhomologous end joining
Process	GO:0036297	interstrand cross-link repair
Process	GO:0031848	protection from non-homologous end joining at telomere

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.08	0.96
Trophozoite	not expressed	0.43	0.74
Schizont	not expressed	0.06	0.97
Gametocyte	not expressed	0.66	0.64

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1474300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.997 | MFS: -1.161 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1301900)

Relative Growth Rate: 0.88 ± 0.21
Confidence: 4.52

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1301900)

Modification: Disrupted | RMgm-3406

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IK95

"Best" AlphaFill ligand hit: 9F2 (ceftriaxone, Local RMSD=0.31) with 5NZW (Global RMSD=0.85)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.5.2.6	beta-lactamase	I2KClDTTEDTATCEPZnCl2CdCl2thiolsRRGHYYAAGHYYvitexinYM09330...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q96SD1	Protein artemis	Homo sapiens	OrthoDB	MLS000027148MLS000071484MLS000080795MLS000040057...

Orthology Information

Ortholog Group (OrthoMCL): OG6_102812

Most Similar Human Ortholog: Q6PJP8

TM-align score: 0.37 | RMSD: 2.70

Seq Identity: 0.32 | Length: 333 / 860

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000198924	DNA cross-link repair 1A

Protein Information

Protein Length: 860 | Molecular Weight (kDa): 102.35

UniProt ID(s): Q8IK95

PDB ID(s): None

Isoelectric Point: 8.92

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001279	Metallo-beta-lactamase
InterPro	IPR011084	DNA repair metallo-beta-lactamase
InterPro	IPR036866	Ribonuclease Z/Hydroxyacylglutathione hydrolase-like
PFam	PF07522	DNA repair metallo-beta-lactamase
Superfamily	SSF56281	Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	9	53	22	59
disruptive	45	186	101	216
missense	32	164	95	186

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	19	98	33	62
disruptive	90	400	148	281
missense	53	347	124	189

PlasmoDB Total SNPs: 133

Non-coding: 44 | Synonymous: 51 | Nonsynonymous: 37 | Stop Codon: 1