Genome location: Pf3D7_14_v3:3,106,516..3,111,987(+)
Genome classification: Core
Product Description: LCCL domain-containing protein
SignalP Peptide: MHHLLFIIWYIILNYYVSG
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0044312 | crystalloid |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0020003 | symbiont-containing vacuole |
| Function | GO:0005515 | protein binding |
| Function | GO:0044877 | protein-containing complex binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.02 | 0.99 |
| Trophozoite | not expressed | 0.03 | 0.98 |
| Schizont | not expressed | 0.62 | 0.66 |
| Gametocyte | not expressed | 0.71 | 0.67 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1475500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.188 | #Insertions: 5
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1300700)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1300700)
Modification: Disrupted | RMgm-4273
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IK83
"Best" AlphaFill ligand hit: 1PG (2-(2-{2-[2-(2-methoxy-ethoxy)-ethoxy]-ethoxy}-ethoxy)-ethanol, Local RMSD=0.03) with 3A21 (Global RMSD=14.75)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 70 | 38 | 60 |
| disruptive | 16 | 77 | 57 | 147 |
| missense | 16 | 76 | 55 | 135 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 24 | 132 | 45 | 67 |
| disruptive | 28 | 182 | 105 | 237 |
| missense | 25 | 158 | 98 | 186 |
PlasmoDB Total SNPs: 80
Non-coding: 13 | Synonymous: 40 | Nonsynonymous: 27 | Stop Codon: 0
Protein Length: 1620 | Molecular Weight (kDa): 184.875
UniProt ID(s): Q8IK83
PDB ID(s): None
Isoelectric Point: 4.9
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000421 | Coagulation factor 5/8 C-terminal domain |
| InterPro | IPR004043 | LCCL domain |
| InterPro | IPR008979 | Galactose-binding-like domain superfamily |
| InterPro | IPR035992 | Ricin B-like lectins |
| InterPro | IPR036056 | Fibrinogen-like, C-terminal |
| InterPro | IPR036609 | LCCL domain superfamily |
| PFam | PF00754 | Coagulation factor 5/8 C-terminal domain |
| PFam | PF03815 | LCCL domain |
| Superfamily | SSF49785 | Galactose-binding-like domain superfamily |
| Superfamily | SSF50370 | Ricin B-like lectins |
| Superfamily | SSF56496 | Fibrinogen-like, C-terminal |
| Superfamily | SSF69848 | LCCL domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 15184503 | A multidomain adhesion protein family expressed in Plasmodium falciparum isessential for transmission to the mosquito. | Pradel G, Hayton K, ..., Templeton TJ | 10.1084/jem.20031274 |
| 19304662 | Sexual stage adhesion proteins form multi-protein complexes in the malariaparasite Plasmodium falciparum. | Simon N, Scholz SM, ..., Pradel G | 10.1074/jbc.M808472200 |
| 19932717 | Plasmodium berghei crystalloids contain multiple LCCL proteins. | Saeed S, Carter V, Tremp AZ, Dessens JT | https://pubmed.ncbi.nlm.nih.gov/19932717/ |
| 26537493 | A WD40-repeat protein unique to malaria parasites associates with adhesionprotein complexes and is crucial for blood stage progeny. | von Bohl A, Kuehn A, ..., Pradel G | 10.1186/s12936-015-0967-x |
| 36479121 | Hijacking the human complement inhibitor C4b-binding protein by the sporozoitestage of the Plasmodium falciparum parasite | Khattab A, Rezola M, ..., Meri S | 10.3389/fimmu.2022.1051161 |