PF3D7_1475700

Genome location: Pf3D7_14_v3:3,117,392..3,118,951(+)

Genome classification: Core

Function and Localization

Product Description: tubulin epsilon chain, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005874	microtubule
Function	GO:0005525	GTP binding
Function	GO:0005200	structural constituent of cytoskeleton
Function	GO:0005198	structural molecule activity
Process	GO:0000226	microtubule cytoskeleton organization
Process	GO:0007018	microtubule-based movement
Process	GO:0007017	microtubule-based process
Process	GO:0000278	mitotic cell cycle

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	not expressed	0.04	0.98
Trophozoite	not expressed	0.03	0.98
Schizont	not expressed	0.03	0.98
Gametocyte	expressed	0.24	0.86

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1475700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.991 | MFS: -2.687 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1300500)

Relative Growth Rate: 0.95 ± 0.08
Confidence: 6.39

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1300500)

Modification: Disrupted | RMgm-3399

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IK81

"Best" AlphaFill ligand hit: GDP (guanosine-5'-diphosphate, Local RMSD=0.23) with 5NJH (Global RMSD=3.55)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_105183

Most Similar Human Ortholog: S4R3K3

TM-align score: 0.91 | RMSD: 1.75

Seq Identity: 0.42 | Length: 211 / 519

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000074935	tubulin epsilon 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	7	7	16
disruptive	4	26	8	44
missense	4	25	8	35

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	14	10	23
disruptive	10	54	36	94
missense	7	43	27	54

PlasmoDB Total SNPs: 22

Non-coding: 1 | Synonymous: 16 | Nonsynonymous: 4 | Stop Codon: 1

Protein Information

Protein Length: 519 | Molecular Weight (kDa): 59.61

UniProt ID(s): Q8IK81

PDB ID(s): None

Isoelectric Point: 7.26

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000217	Tubulin
InterPro	IPR003008	Tubulin/FtsZ, GTPase domain
InterPro	IPR004057	Epsilon tubulin
InterPro	IPR008280	Tubulin/FtsZ, C-terminal
InterPro	IPR017975	Tubulin, conserved site
InterPro	IPR018316	Tubulin/FtsZ, 2-layer sandwich domain
InterPro	IPR036525	Tubulin/FtsZ, GTPase domain superfamily
PFam	PF00091	Tubulin/FtsZ, GTPase domain
PFam	PF03953	Tubulin/FtsZ, 2-layer sandwich domain
Superfamily	SSF52490	Tubulin/FtsZ, GTPase domain superfamily
Superfamily	SSF55307	Tubulin/FtsZ, C-terminal