Genome location: Pf3D7_14_v3:3,156,572..3,158,056(+)
Genome classification: Core
Product Description: lysophospholipase LPL1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.1.5 (Lysophospholipase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0016020 | membrane |
| Component | GO:0005739 | mitochondrion |
| Component | GO:0031982 | vesicle |
| Function | GO:0016787 | hydrolase activity |
| Function | GO:0016298 | lipase activity |
| Function | GO:0004622 | lysophospholipase activity |
| Process | GO:0006638 | neutral lipid metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.10 | 0.95 |
| Trophozoite | expressed | 0.23 | 0.86 |
| Schizont | expressed | 0.11 | 0.93 |
| Gametocyte | expressed | 0.73 | 0.62 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1476700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.999 | MFS: -2.442 | #Insertions: 2
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1220300)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IK69
"Best" AlphaFill ligand hit: PMS (phenylmethanesulfonic acid, Local RMSD=0.12) with 3RLI (Global RMSD=3.61)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.1.5 | lysophospholipase | NEMSDSEDTAPCMBEGTAPMSFDMSOCaCl2FeCl3xyleneethanolsulfite... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100231
Most Similar Human Ortholog: A0A3B3ITT3
TM-align score: 0.86 | RMSD: 1.97
Seq Identity: 0.19 | Length: 243 / 353
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000074416 | monoglyceride lipase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 11 | 9 | 16 |
| disruptive | 2 | 33 | 25 | 42 |
| missense | 2 | 33 | 23 | 38 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 26 | 16 | 17 |
| disruptive | 13 | 81 | 45 | 88 |
| missense | 10 | 70 | 34 | 55 |
PlasmoDB Total SNPs: 24
Non-coding: 17 | Synonymous: 4 | Nonsynonymous: 3 | Stop Codon: 0
Protein Length: 353 | Molecular Weight (kDa): 41.35
UniProt ID(s): Q8IK69
PDB ID(s): None
Isoelectric Point: 9.01
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006494 | Plasmodium subtelomeric PST-A |
| InterPro | IPR022742 | Serine aminopeptidase, S33 |
| InterPro | IPR029058 | Alpha/Beta hydrolase fold |
| PFam | PF12146 | Serine aminopeptidase, S33 |
| Superfamily | SSF53474 | Alpha/Beta hydrolase fold |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34380472 | An essential vesicular-trafficking phospholipase mediates neutral lipid synthesisand contributes to hemozoin formation in Plasmodium falciparum. | Asad M, Yamaryo-Botte Y, ..., Mohmmed A | 10.1186/s12915-021-01042-z |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 35817856 | Rescuing yeast from cell death enables overproduction of fatty acids from solemethanol | Gao J, Li Y, Yu W, Zhou YJ | 10.1038/s42255-022-00601-0 |
| 25014274 | Identification of a phospholipase B encoded by the LPL1 gene in Saccharomycescerevisiae | Selvaraju K, Rajakumar S, Nachiappan V | 10.1016/j.bbalip.2014.06.013 |