PF3D7_1476800 (LPL3)

Genome location: Pf3D7_14_v3:3,159,438..3,160,837(+)

Genome classification: Core

Function and Localization

Product Description: lysophospholipase LPL3

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.1.5 (Lysophospholipase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005829	cytosol
Component	GO:0016020	membrane
Component	GO:0020006	symbiont-containing vacuolar membrane network
Component	GO:0020003	symbiont-containing vacuole
Function	GO:0016787	hydrolase activity
Function	GO:0016298	lipase activity
Process	GO:0055088	lipid homeostasis
Process	GO:0009410	response to xenobiotic stimulus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.30	0.84
Trophozoite	expressed	0.19	0.88
Schizont	expressed	0.15	0.90
Gametocyte	expressed	0.15	0.91

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1476800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.149 | MFS: -3.317 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1220300)

Relative Growth Rate: 1.09 ± 0.18
Confidence: 4.84

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IK68

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.1.5	lysophospholipase	NEMSDSEDTAPCMBEGTAPMSFDMSOCaCl2FeCl3xyleneethanolsulfite...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100231

Most Similar Human Ortholog: A0A3B3ITT3

TM-align score: 0.85 | RMSD: 2.12

Seq Identity: 0.18 | Length: 243 / 371

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000074416	monoglyceride lipase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	14	17	20
disruptive	8	42	22	35
missense	7	38	21	31

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	33	14	20
disruptive	17	83	36	78
missense	13	70	26	43

PlasmoDB Total SNPs: 47

Non-coding: 18 | Synonymous: 20 | Nonsynonymous: 9 | Stop Codon: 0

Protein Information

Protein Length: 371 | Molecular Weight (kDa): 42.714

UniProt ID(s): Q8IK68

PDB ID(s): None

Isoelectric Point: 8.93

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006494	Plasmodium subtelomeric PST-A
InterPro	IPR022742	Serine aminopeptidase, S33
InterPro	IPR029058	Alpha/Beta hydrolase fold
PFam	PF12146	Serine aminopeptidase, S33
Superfamily	SSF53474	Alpha/Beta hydrolase fold