Genome location: Pf3D7_14_v3:3,253,655..3,256,879(+)
Genome classification: Core
Product Description: acyl-CoA synthetase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.2.1.3 (Long-chain-fatty-acid--CoA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0030430 | host cell cytoplasm |
| Component | GO:0016020 | membrane |
| Function | GO:0004467 | long-chain fatty acid-CoA ligase activity |
| Process | GO:0006631 | fatty acid metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.23 | 0.91 |
| Trophozoite | not expressed | 0.03 | 0.98 |
| Schizont | expressed | 0.03 | 0.98 |
| Gametocyte | not expressed | 0.04 | 0.98 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1479000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.129 | MFS: -3.06 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1453300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0311800)
Modification: Disrupted | RMgm-1796
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q7KQL7
"Best" AlphaFill ligand hit: APC (alpha,beta-methyleneadenosine-5'-triphosphate, Local RMSD=0.38) with 6MFW (Global RMSD=3.28)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.2.1.3 | long-chain-fatty-acid-CoA ligase | ATPAMPNEMSDSEDTAsaltsoleateGW1929Brij 58Tween 80naproxentriacsin... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100152
Most Similar Human Ortholog: B7Z3Z9
TM-align score: 0.90 | RMSD: 2.68
Seq Identity: 0.22 | Length: 515 / 820
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000151726 | acyl-CoA synthetase long chain family member 1 |
| ENSG00000164398 | acyl-CoA synthetase long chain family member 6 |
| ENSG00000197142 | acyl-CoA synthetase long chain family member 5 |
| ENSG00000281938 | novel transcript |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 31 | 12 | 43 |
| disruptive | 49 | 152 | 89 | 167 |
| missense | 38 | 128 | 73 | 120 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 20 | 47 | 16 | 41 |
| disruptive | 85 | 257 | 110 | 191 |
| missense | 70 | 206 | 70 | 103 |
PlasmoDB Total SNPs: 142
Non-coding: 34 | Synonymous: 40 | Nonsynonymous: 67 | Stop Codon: 1
Protein Length: 820 | Molecular Weight (kDa): 94.874
UniProt ID(s): Q25708, Q7KQL7, Q9TY41, Q9U5A6, Q9U8K3
PDB ID(s): None
Isoelectric Point: 9.04
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000873 | AMP-dependent synthetase/ligase |
| InterPro | IPR020845 | AMP-binding, conserved site |
| PFam | PF00501 | AMP-dependent synthetase/ligase |
| Superfamily | SSF56801 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10438606 | The cloning and expression of Pfacs1, a Plasmodium falciparum fatty acyl coenzymeA synthetase-1 targeted to the host erythrocyte cytoplasm. | Matesanz F, Duran-Chica I, Alcina A | 10.1006/jmbi.1999.2964 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |