About Gene List

PF3D7_API02700 (RPS12)

Genome location: Pf3D7_API_v3:7,559..7,927(+)

Genome classification: Apicoplast

Function and Localization

Product Description: apicoplast ribosomal protein S12

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005840 ribosome
Function GO:0003735 structural constituent of ribosome
Process GO:0006412 translation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite no data N/A N/A
Ring no data nd nd
Trophozoite no data nd nd
Schizont no data nd nd
Gametocyte no data nd nd

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PFC10_API0030

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: N/A

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0613400)

  • Relative Growth Rate: 0.08 ± 0.05
  • Confidence: 7.48

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q25818

"Best" AlphaFill ligand hit: AM2 (4-o-(3alpha-amino-6alpha-((4-amino-4-deoxy-alpha-d-glucopyranosyl)oxy)-2,3,4,5abeta,6,7,8,8aalpha-octahydro-8beta-hydroxy-7beta-(methylamino)pyrano(3,2-b)pyran-2alpha-yl)-2-deoxy-d-streptamine, Local RMSD=0.04) with 4AQY (Global RMSD=0.65)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101451

Most Similar Human Ortholog: O15235

TM-align score: 0.82 | RMSD: 1.44

Seq Identity: 0.42 | Length: 108 / 122

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000128626 mitochondrial ribosomal protein S12
ENSG00000283018 mitochondrial ribosomal protein S12

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

No passing variants found

PlasmoDB Total SNPs: 8

Non-coding: 0 | Synonymous: 6 | Nonsynonymous: 2 | Stop Codon: 0

Protein Information

Protein Length: 122 | Molecular Weight (kDa): 13.944

UniProt ID(s): Q25818

PDB ID(s): None

Isoelectric Point: 11.19

Protein Domain Annotations:

Source Family ID Description
InterPro IPR005679 Ribosomal protein S12, bacterial-type
InterPro IPR006032 Ribosomal protein S12/S23
InterPro IPR012340 Nucleic acid-binding, OB-fold
PFam PF00164 Ribosomal protein S12/S23
Superfamily SSF50249 Nucleic acid-binding, OB-fold

Associated Publications

PMID Title Authors DOI/Link
8757284 Complete gene map of the plastid-like DNA of the malaria parasite Plasmodiumfalciparum. Wilson RJ, Denny PW, ..., Williamson DH 10.1006/jmbi.1996.0449
27458998 Transcripts in the Plasmodium Apicoplast Undergo Cleavage at tRNAs and Editing,and Include Antisense Sequences. Nisbet RER, Kurniawan DP, Bowers HD, Howe CJ 10.1016/j.protis.2016.06.003