PF3D7_API02900 (TUFA)

Genome location: Pf3D7_API_v3:8,417..9,649(+)

Genome classification: Apicoplast

Function and Localization

Product Description: elongation factor Tu

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast
Component	GO:0005739	mitochondrion
Function	GO:0003746	translation elongation factor activity
Process	GO:0070125	mitochondrial translational elongation
Process	GO:0006414	translational elongation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	no data	N/A	N/A
Ring	no data	nd	nd
Trophozoite	no data	nd	nd
Schizont	no data	nd	nd
Gametocyte	no data	nd	nd

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PFC10_API0028

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: N/A

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1345600)

Relative Growth Rate: 0.15 ± 0.19
Confidence: 4.68

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q25820

"Best" AlphaFill ligand hit: 5GP (guanosine-5'-monophosphate, Local RMSD=0.30) with 4AC9 (Global RMSD=5.30)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100780

Most Similar Human Ortholog: P49411

TM-align score: 0.93 | RMSD: 1.51

Seq Identity: 0.41 | Length: 395 / 410

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000178952	Tu translation elongation factor, mitochondrial

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

No passing variants found

PlasmoDB Total SNPs: 35

Non-coding: 0 | Synonymous: 25 | Nonsynonymous: 8 | Stop Codon: 2

Protein Information

Protein Length: 410 | Molecular Weight (kDa): 46.635

UniProt ID(s): A0A140KXW6, Q25820

PDB ID(s): None

Isoelectric Point: 8.41

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000795	Transcription factor, GTP-binding domain
InterPro	IPR004160	Translation elongation factor EFTu/EF1A, C-terminal
InterPro	IPR004161	Translation elongation factor EFTu-like, domain 2
InterPro	IPR004541	Translation elongation factor EFTu/EF1A, bacterial/organelle
InterPro	IPR005225	Small GTP-binding protein domain
InterPro	IPR009000	Translation protein, beta-barrel domain superfamily
InterPro	IPR009001	Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
InterPro	IPR031157	Tr-type G domain, conserved site
InterPro	IPR033720	Elongation factor Tu, domain 2
PFam	PF00009	Transcription factor, GTP-binding domain
PFam	PF03143	Translation elongation factor EFTu/EF1A, C-terminal
PFam	PF03144	Translation elongation factor EFTu-like, domain 2
Superfamily	SSF50447	Translation protein, beta-barrel domain superfamily
Superfamily	SSF50465	Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase