About Gene List

PF3D7_API04300 (rpoC1)

Genome location: Pf3D7_API_v3:17,247..18,974(-)

Genome classification: Apicoplast

Function and Localization

Product Description: DNA-directed RNA polymerase subunit beta', putative

SignalP Peptide: N/A

# Transmembrane Domains: 3

EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020011 apicoplast

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite no data N/A N/A
Ring no data nd nd
Trophozoite no data nd nd
Schizont no data nd nd
Gametocyte no data nd nd

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PFC10_API0016

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: N/A

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1344000)

  • Relative Growth Rate: 0.21 ± 0.20
  • Confidence: 4.63

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: P21422

"Best" AlphaFill ligand hit: POP (pyrophosphate 2-, Local RMSD=0.08) with 5VO8 (Global RMSD=2.26)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
2.7.7.6 DNA-directed RNA polymerase BRENDA SOAP query unsuccessful

Orthology to BindingDB Entries:

UniProt Protein Name Species Homology Source BindingDB Ligands
P0A8T7 DNA-directed RNA polymerase subunit beta' OrthoMCL, OrthoMCL BLAST CHEMBL3228736CHEMBL3228737Actinomycin D analogue

Orthology Information

Ortholog Group (OrthoMCL): OG6_100210

Most Similar Human Ortholog: O14802

TM-align score: 0.69 | RMSD: 4.51

Seq Identity: 0.19 | Length: 458 / 575

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000148606 RNA polymerase III subunit A
ENSG00000181222 RNA polymerase II subunit A
ENSG00000284832 RNA polymerase II subunit A

Protein Information

Protein Length: 575 | Molecular Weight (kDa): 69.406

UniProt ID(s): A0A140KXX4, P21422

PDB ID(s): None

Isoelectric Point: 10.29

Protein Domain Annotations:

Source Family ID Description
InterPro IPR000722 RNA polymerase, alpha subunit
InterPro IPR006592 RNA polymerase, N-terminal
InterPro IPR007080 RNA polymerase Rpb1, domain 1
PFam PF00623 RNA polymerase, alpha subunit
PFam PF04997 RNA polymerase Rpb1, domain 1
Superfamily SSF64484 N/A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

No passing variants found

PlasmoDB Total SNPs: 30

Non-coding: 0 | Synonymous: 26 | Nonsynonymous: 4 | Stop Codon: 0

Associated Publications

PMID Title Authors DOI/Link
8757284 Complete gene map of the plastid-like DNA of the malaria parasite Plasmodiumfalciparum. Wilson RJ, Denny PW, ..., Williamson DH 10.1006/jmbi.1996.0449
27458998 Transcripts in the Plasmodium Apicoplast Undergo Cleavage at tRNAs and Editing,and Include Antisense Sequences. Nisbet RER, Kurniawan DP, Bowers HD, Howe CJ 10.1016/j.protis.2016.06.003
36818825 The complete chloroplast genome of Hibiscus syriacus using long-read sequencing:Comparative analysis to examine the evolution of the tribe Hibisceae Koo H, Shin AY, Hong S, Kim YM 10.3389/fpls.2023.1111968
36693990 Complete chloroplast genome molecular structure, comparative and phylogeneticanalyses of Sphaeropteris lepifera of Cyatheaceae family: a tree fern from China Hu Q, Qian R, ..., Zheng J 10.1038/s41598-023-28432-3
36548605 Genetic Diversity of Microcystin Producers (Cyanobacteria) and Microcystin Congeners in Aquatic Resources across Africa: A Review Paper Chia MA, Ameh I, ..., Lorenzi AS 10.3390/toxics10120772
37298153 The ATP Synthase gamma Subunit ATPC1 Regulates RNA Editing in Chloroplasts Ni J, Song W, ..., Zhao X 10.3390/ijms24119203