Genome location: Pf3D7_API_v3:17,247..18,974(-)
Genome classification: Apicoplast
Product Description: DNA-directed RNA polymerase subunit beta', putative
SignalP Peptide: N/A
# Transmembrane Domains: 3
EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)
Curated GO (PlasmoDB):
Type | GO Term | Name |
---|---|---|
Component | GO:0020011 | apicoplast |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
Stage | LR class | MCA mean | MCA prop. zeros |
---|---|---|---|
Sporozoite | no data | N/A | N/A |
Ring | no data | nd | nd |
Trophozoite | no data | nd | nd |
Schizont | no data | nd | nd |
Gametocyte | no data | nd | nd |
More info:
Old (Pf3D7v3) Gene ID: PFC10_API0016
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: N/A
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1344000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: P21422
"Best" AlphaFill ligand hit: POP (pyrophosphate 2-, Local RMSD=0.08) with 5VO8 (Global RMSD=2.26)
BRENDA EC Inhibitors:
EC # | Name | EC Inhibitors |
---|---|---|
2.7.7.6 | DNA-directed RNA polymerase | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
---|---|---|---|---|
P0A8T7 | DNA-directed RNA polymerase subunit beta' | OrthoMCL, OrthoMCL BLAST | CHEMBL3228736CHEMBL3228737Actinomycin D analogue |
Ortholog Group (OrthoMCL): OG6_100210
Most Similar Human Ortholog: O14802
TM-align score: 0.69 | RMSD: 4.51
Seq Identity: 0.19 | Length: 458 / 575
All Human Orthologs (OrthoMCL):
Gene ID | Description |
---|---|
ENSG00000148606 | RNA polymerase III subunit A |
ENSG00000181222 | RNA polymerase II subunit A |
ENSG00000284832 | RNA polymerase II subunit A |
Protein Length: 575 | Molecular Weight (kDa): 69.406
UniProt ID(s): A0A140KXX4, P21422
PDB ID(s): None
Isoelectric Point: 10.29
Protein Domain Annotations:
Source | Family ID | Description |
---|---|---|
InterPro | IPR000722 | RNA polymerase, alpha subunit |
InterPro | IPR006592 | RNA polymerase, N-terminal |
InterPro | IPR007080 | RNA polymerase Rpb1, domain 1 |
PFam | PF00623 | RNA polymerase, alpha subunit |
PFam | PF04997 | RNA polymerase Rpb1, domain 1 |
Superfamily | SSF64484 | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 30
Non-coding: 0 | Synonymous: 26 | Nonsynonymous: 4 | Stop Codon: 0
PMID | Title | Authors | DOI/Link |
---|---|---|---|
8757284 | Complete gene map of the plastid-like DNA of the malaria parasite Plasmodiumfalciparum. | Wilson RJ, Denny PW, ..., Williamson DH | 10.1006/jmbi.1996.0449 |
27458998 | Transcripts in the Plasmodium Apicoplast Undergo Cleavage at tRNAs and Editing,and Include Antisense Sequences. | Nisbet RER, Kurniawan DP, Bowers HD, Howe CJ | 10.1016/j.protis.2016.06.003 |
36818825 | The complete chloroplast genome of Hibiscus syriacus using long-read sequencing:Comparative analysis to examine the evolution of the tribe Hibisceae | Koo H, Shin AY, Hong S, Kim YM | 10.3389/fpls.2023.1111968 |
36693990 | Complete chloroplast genome molecular structure, comparative and phylogeneticanalyses of Sphaeropteris lepifera of Cyatheaceae family: a tree fern from China | Hu Q, Qian R, ..., Zheng J | 10.1038/s41598-023-28432-3 |
36548605 | Genetic Diversity of Microcystin Producers (Cyanobacteria) and Microcystin Congeners in Aquatic Resources across Africa: A Review Paper | Chia MA, Ameh I, ..., Lorenzi AS | 10.3390/toxics10120772 |
37298153 | The ATP Synthase gamma Subunit ATPC1 Regulates RNA Editing in Chloroplasts | Ni J, Song W, ..., Zhao X | 10.3390/ijms24119203 |