Genome location: Pf3D7_API_v3:18,977..22,051(-)
Genome classification: Apicoplast
Product Description: DNA-directed RNA polymerase subunit beta, putative
SignalP Peptide: N/A
# Transmembrane Domains: 5
EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | no data | N/A | N/A |
| Ring | no data | nd | nd |
| Trophozoite | no data | nd | nd |
| Schizont | no data | nd | nd |
| Gametocyte | no data | nd | nd |
More info:
Old (Pf3D7v3) Gene ID: PFC10_API0015
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: N/A
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0605200)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: P21421
"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=0.02) with 5D4C (Global RMSD=3.25)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.6 | DNA-directed RNA polymerase | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P0A8V2 | DNA-directed RNA polymerase subunit beta | OrthoMCL, OrthoMCL BLAST | MRL-436RIFAMYCINMRL-436RIFAMYCIN... | |
| P9WGY9 | DNA-directed RNA polymerase subunit beta | Mycobacterium tuberculosis | OrthoMCL, OrthoMCL BLAST | CHEMBL1911286CHEMBL1911287CHEMBL1911289CHEMBL1911296... |
| A0A0E2LB94 | DNA-directed RNA polymerase subunit beta | Escherichia coli | OrthoMCL BLAST | MLS000070992MLS000034979CHEMBL2046886 |
| Q3YUZ7 | DNA-directed RNA polymerase subunit beta [S531L] | OrthoMCL BLAST | MRL-436MRL-436MRL-436 |
Ortholog Group (OrthoMCL): OG6_100190
Most Similar Human Ortholog: P30876
TM-align score: 0.65 | RMSD: 5.66
Seq Identity: 0.18 | Length: 789 / 1024
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000013503 | RNA polymerase III subunit B |
| ENSG00000047315 | RNA polymerase II subunit B |
Protein Length: 1024 | Molecular Weight (kDa): 122.186
UniProt ID(s): A0A140KXX5, P21421
PDB ID(s): None
Isoelectric Point: 10.03
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR007120 | DNA-directed RNA polymerase, subunit 2, hybrid-binding domain |
| InterPro | IPR007121 | RNA polymerase, beta subunit, conserved site |
| InterPro | IPR007641 | RNA polymerase Rpb2, domain 7 |
| InterPro | IPR007645 | RNA polymerase Rpb2, domain 3 |
| InterPro | IPR015712 | DNA-directed RNA polymerase, subunit 2 |
| PFam | PF00562 | DNA-directed RNA polymerase, subunit 2, hybrid-binding domain |
| PFam | PF04560 | RNA polymerase Rpb2, domain 7 |
| PFam | PF04565 | RNA polymerase Rpb2, domain 3 |
| Superfamily | SSF64484 | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 31
Non-coding: 0 | Synonymous: 23 | Nonsynonymous: 8 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 7808472 | Phylogenetic analysis of the rpoB gene from the plastid-like DNA of Plasmodiumfalciparum. | Gardner MJ, Goldman N, ..., Wilson RJ | 10.1016/0166-6851(94)90149-x |
| 8757284 | Complete gene map of the plastid-like DNA of the malaria parasite Plasmodiumfalciparum. | Wilson RJ, Denny PW, ..., Williamson DH | 10.1006/jmbi.1996.0449 |
| 38392836 | Phenotypic and Genotypic Drug Resistance of Mycobacterium tuberculosis StrainsIsolated from HIV-Infected Patients from a Third-Level Public Hospital in Mexico | Valencia-Trujillo D, Avila-Trejo AM, ..., Cerna-Cortes JF | 10.3390/pathogens13020098 |
| 38381041 | Autotrophic growth of Escherichia coli is achieved by a small number of genetic changes | Ben Nissan R, Milshtein E, ..., Milo R | 10.7554/eLife.88793 |
| 38360762 | Selection and validation of reference genes suitable for gene expression analysis by Reverse Transcription Quantitative real-time PCR in Acinetobacter baumannii | de Oliveira PAA, Baboghlian J, ..., Ferraz LFC | 10.1038/s41598-024-51499-5 |
| 38391520 | Implementation of Whole Genome Sequencing of Tuberculosis Isolates in a Referral Center in Rome: Six Years' Experience in Characterizing Drug-Resistant TB and Disease Transmission | Cannas A, Butera O, ..., Girardi E | 10.3390/antibiotics13020134 |
| 38399727 | Mutational Dynamics Related to Antibiotic Resistance in M. tuberculosis Isolates from Serial Samples of Patients with Tuberculosis and Type 2 Diabetes Mellitus | Bermudez-Hernandez GA, Perez-Martinez D, ..., Zenteno-Cuevas R | 10.3390/microorganisms12020324 |
| 38401221 | Genetic diversity of Bartonella rpoB haplotypes in domestic cats from Chile | Sepulveda-Garcia P, Jara R, ..., Muller A | 10.1016/j.cimid.2024.102150 |